Erg K+ channels modulate contractile activity in the bovine epididymal duct

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Marco MeweC K Bauer

Abstract

The expression and functional role of ether-à-go-go-related gene (erg) K+ channels were examined in the bovine epididymal duct. Sperm transit through the epididymal duct relies on spontaneous phasic contractions (SC) of the peritubular smooth muscle wall. Isometric tension studies revealed SC-enhancing effects of the erg channel blockers E-4031, dofetilide, cisapride, and haloperidol and SC-suppressing effects of the activator NS-1643. In the corpus epididymidis, EC50 values of 32 nM and 8.3 microM were determined for E-4031 and NS-1643, respectively. E-4031 was also able to elicit contraction in epithelium-denuded corpus segments, which lacked SC. In the cauda region, E-4031 and NS-1643 exerted effects on agonist-induced contraction similar to those observed in the proximal duct. Experiments with nifedipine and thapsigargin suggested that the excitatory effects of E-4031 depended mainly on external calcium influx and not on intracellular calcium release. Western blot and RT-PCR assays revealed the expression of both, erg1a and erg1b, in all duct regions. Because erg1b appears to predominate in the epididymal duct, patch-clamp experiments were performed on heterologously expressed erg1b channels to investigate the sensitivity o...Continue Reading

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Citations

Nov 19, 2009·Histochemistry and Cell Biology·Rolando CarrisozaLaura I Escobar
Feb 1, 2011·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Mar 26, 2009·Pflügers Archiv : European journal of physiology·Karoline EinarsenNicole Schmitt
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Sep 20, 2012·Physiological Reviews·Jamie I VandenbergAdam P Hill

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