Erianin suppresses hepatocellular carcinoma cells through down-regulation of PI3K/AKT, p38 and ERK MAPK signaling pathways.

Bioscience Reports
Liang YangZhenshun Song

Abstract

Hepatocellular carcinoma (HCC) is the dominant pathological type of primary liver cancer and no effective methods are available for its treatment. Erianin is a natural product extracted from Dendrobium, which possesses multiple pharmacological activities, including antioxidative and antitumor activity. To evaluate the anti-HCC activities of erianin and explore its underlying mechanism. MTT assay and Crystal Violet staining assay were used to select the non-toxic concentrations for the subsequent experiments. The colony formation assay and PCNA fluorescent staining were used to investigate the antiproliferative effects of erianin on human SMMC-7721 and HepG2 cells. Wound healing and transwell test were used to analyze cell migration and invasion. Caspase3 and Tunel staining were used to detect apoptosis. Western blot was used to examine the expression levels of proteins associated with invasion and key proteins in the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt), p38 and ERK mitogen-activated protein kinase (MAPK) signaling pathways. Erianin inhibited HCC cell proliferation in a dose-dependent manner. Decreased migration rate and invaded cells were observed with erianin supplement. The expression of invasion-associa...Continue Reading

References

Jun 16, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y-Q GongZ-T Wang
Apr 1, 2014·International Journal of Molecular Medicine·Li ZhangJuan Peng
Dec 3, 2014·Surgical Oncology Clinics of North America·Kelly J LafaroTimothy M Pawlik
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Dec 12, 2018·BMC Complementary and Alternative Medicine·Yayun QianYanqing Liu
Jan 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lulu XieMingdong Zhao
Feb 15, 2019·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mei-Fen ChenI-Min Liu
Feb 23, 2019·Journal of Cellular and Molecular Medicine·Lulu XieMingdong Zhao
Apr 21, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Hai ZhangXiao-Fei Shen
May 9, 2019·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Tianqiang JinFeng Xu

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
fluorescence staining

Software Mentioned

SPSS18

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis