ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells

Stem Cell Research & Therapy
Sami G Almalki, Devendra K Agrawal

Abstract

Cell-based therapy that can rejuvenate the endothelium with stimulated adipose-derived mesenchymal stem cells (AMSCs) is a promising therapeutic strategy for the re-endothelialization of denuded arteries at the stenting site. Previously, we have shown that silencing of MMP-2 and MMP-14 inhibits vascular endothelial growth factor receptor type 2 (VEGFR2) cleavage, and induces differentiation of AMSCs toward the endothelial cell (EC) lineage. In this study, we examined the underlying signaling pathways that regulate differentiation of AMSCs to ECs in vitro through VEGFR2. AMSCs were isolated from porcine abdominal adipose tissue. The isolated AMSCs were characterized by positive expression of CD29, CD44, and CD90 and negative expression of CD11b and CD45. The isolated MSCs were transfected with siRNA to silence MMP-2, MMP-14, and angiotensin receptor 2 (ATR2). Cells were suspended either in endothelial basal media (EBM) or endothelial growth media (EGM) with various treatments. Flow cytometry was performed to examine the expression of EC markers, and western blot analysis was performed to examine the expression and activity of various kinases. Scratch assay was performed to examine the cell migration. Data were analyzed by ANOVA ...Continue Reading

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Citations

Mar 31, 2018·Journal of Cellular Physiology·Jacopo PizzicannellaOriana Trubiani
Oct 27, 2017·Stem Cell Research & Therapy·Lufeng LiXiaohui Zhao
Aug 12, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Guoku HuShilpa Buch
Dec 20, 2019·Angiogenesis·M di SommaS Mitola
Apr 29, 2020·Cell Death & Disease·Hong WeiYuefeng Li
Sep 17, 2018·Cell Communication and Signaling : CCS·Yan WuJulia Li Zhong
Feb 13, 2021·Frontiers in Cardiovascular Medicine·Weili ShiKeji Chen
Jul 16, 2021·Photobiomodulation, Photomedicine, and Laser Surgery·Shi-Jie HuangYu-Jung Cheng

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Methods Mentioned

BETA
density gradient centrifugation
Flow Cytometry
transfection
transfections

Software Mentioned

Enspire Manager
Image Lab

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