ERK1/2 and HIF1α Are Involved in Antiangiogenic Effect of Polyphenols-Enriched Fraction from Chilean Propolis

Evidence-based Complementary and Alternative Medicine : ECAM
Alejandro CuevasLuis A Salazar

Abstract

Propolis has been shown to modulate the angiogenesis in both in vitro and in vivo models. Thus, we aimed to evaluate the antiangiogenic properties of an ethanolic extract of Chilean propolis (EEP) and Pinocembrin (Pn). Migration, formation of capillary-like structures of endothelial cells, and sprouting from rat aortic rings were used to assess the antiangiogenic properties of EEP or Pn. In addition, microRNAs and VEGFA mRNA expression were studied by qPCR. ERK1/2 phosphorylation and HIF1α stabilization were assessed by western blot. EEP or Pn attenuated the migration, the capillary-like tube formation, and the sprouting in the in vitro assays. In addition, the activation of HIF1α and ERK1/2 and the VEGFA mRNA expression was significantly inhibited in a dose-dependent manner. In summary, these results suggest that HIF1α and ERK1/2 phosphorylation could be involved in the antiangiogenic effect of Chilean propolis, but more studies are needed to corroborate these findings.

References

Apr 17, 1997·Nature·W Risau
Dec 24, 2002·Fitoterapia·Stefano Castaldo, Francesco Capasso
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Apr 16, 2005·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·M PopovaV Bankova
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Anna-Karin OlssonLena Claesson-Welsh
Jan 30, 2008·European Journal of Clinical Investigation·I D PousaJ P Gisbert
Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Toshiro OhtaKazuhiko Kaji
Dec 10, 2008·Archives of Medical Research·Maryam KeshavarzHamid Reza Mohammadi Motlagh
Dec 10, 2008·Molecular Nutrition & Food Research·Mok-Ryeon AhnToshiro Ohta
Feb 10, 2009·Current Diabetes Reviews·Talia N CrawfordEric P Jablon
Apr 9, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Kazuhiro KunimasaToshiro Ohta
Jun 2, 2009·Seminars in Cancer Biology·Vanessa Baeriswyl, Gerhard Christofori
Aug 29, 2009·BMC Bioinformatics·Stephan PabingerZlatko Trajanoski
Nov 18, 2009·BMC Complementary and Alternative Medicine·Hiroshi IzutaHideaki Hara
Dec 5, 2009·Molecular Nutrition & Food Research·Yuichi ChikaraishiHideaki Hara
Dec 17, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Sandra Aparecida Lima de MouraDenise Carmona Cara
Jul 19, 2011·The Journal of Nutritional Biochemistry·Julio Beltrame DalepraneDulcinéia Saes Parra Abdalla
Dec 27, 2011·Biochemical and Biophysical Research Communications·Runting YinShaohua Gou
Mar 13, 2012·Antioxidants & Redox Signaling·Smitha MalireddyNarasimham L Parinandi
Jan 16, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Joleen Lopes MachadoFlávia Raquel Fernandes do Nascimento
Feb 19, 2013·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Anna M Kurek-GóreckaKatarzyna Pawłowska-Góral
Mar 30, 2013·Microvascular Research·Cristiane MeneghelliMarcelo Maraschin

❮ Previous
Next ❯

Citations

Sep 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Esmerina Tili, Jean-Jacques Michaille
May 26, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Verónica MujicaElba Leiva
Sep 19, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Jorge Jesús VelozLuis A Salazar
Sep 10, 2020·International Journal of Molecular Sciences·Alejandro CuevasDulcineia S P Abdalla
Jan 8, 2021·Biology·Henrique SilvaAntónio Raposo

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

ImageJ
Sigma Plot
QPCR
TScratch

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.