ERK1c regulates Golgi fragmentation during mitosis

The Journal of Cell Biology
Yoav D Shaul, Rony Seger

Abstract

Extracellular signal-regulated kinase 1c (ERK1c) is an alternatively spliced form of ERK1 that is regulated differently than other ERK isoforms. We studied the Golgi functions of ERK1c and found that it plays a role in MEK-induced mitotic Golgi fragmentation. Thus, in late G2 and mitosis of synchronized cells, the expression and activity of ERK1c was increased and it colocalized mainly with Golgi markers. Small interfering RNA of ERK1c significantly attenuated, whereas ERK1c overexpression facilitated, mitotic Golgi fragmentation. These effects were also reflected in mitotic progression, indicating that ERK1c is involved in cell cycle regulation via modulation of Golgi fragmentation. Although ERK1 was activated in mitosis as well, it could not replace ERK1c in regulating Golgi fragmentation. Therefore, MEKs regulate mitosis via all three ERK isoforms, where ERK1c acts specifically in the Golgi, whereas ERK1 and 2 regulate other mitosis-related processes. Thus, ERK1c extends the specificity of the Ras-MEK cascade by activating ERK1/2-independent processes.

References

Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
Aug 23, 1996·The Journal of Biological Chemistry·J N LavoieJ Pouysségur
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·H JaaroR Seger
Sep 23, 1998·Genes & Development·S SifR E Kingston
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J H WrightE G Krebs
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Jul 27, 2002·Annual Review of Cell and Developmental Biology·James Shorter, Graham Warren
Aug 2, 2003·Current Opinion in Cell Biology·Antonino ColanziVivek Malhotra
Jan 23, 2004·Oncogene·Xiaoqi LiuRaymond L Erikson
Aug 3, 2004·Journal of Cell Science·John W Edmunds, Louis C Mahadevan
Jan 26, 2005·Molecular Biotechnology·Dana Chuderland, Rony Seger

❮ Previous
Next ❯

Citations

May 17, 2007·Nature Reviews. Molecular Cell Biology·Martin Lowe, Francis A Barr
May 15, 2007·Oncogene·M RamanM H Cobb
Jul 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Veronika ReitererHesso Farhan
Apr 4, 2008·Molecular Biology of the Cell·Juan Manuel DuranVivek Malhotra
May 16, 2008·Molecular Biology of the Cell·Kai-Hui SunKavita Shah
Apr 25, 2008·Molecular Biology of the Cell·Timothy N Feinstein, Adam D Linstedt
Sep 17, 2010·Molecular Biology of the Cell·Angela PersicoAntonino Colanzi
Dec 18, 2012·Molecular Biology of the Cell·Christine KienzleAngelika Hausser
Jun 22, 2011·Cold Spring Harbor Perspectives in Biology·Yanzhuang Wang, Joachim Seemann
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Marie Cargnello, Philippe P Roux
Jul 23, 2011·Genes & Cancer·Inbal Wortzel, Rony Seger
Jul 4, 2008·PPAR Research·Elke Burgermeister, Rony Seger
Feb 27, 2008·The Journal of Cell Biology·Anastasia BlagoveshchenskayaPeter Mayinger
Nov 3, 2009·FEBS Letters·Angela PersicoAntonino Colanzi
Jun 16, 2010·The Journal of Cell Biology·Hesso FarhanHans-Peter Hauri
Apr 30, 2008·The Journal of Cell Biology·Christopher L de GraffenriedGraham Warren
Jul 8, 2009·The Journal of Cell Biology·Debrup SenguptaAdam D Linstedt
Feb 2, 2016·Current Opinion in Cell Biology·Ehud Wainstein, Rony Seger
Aug 23, 2012·Gene·Olga KelemenStefan Stamm
Jan 7, 2016·Frontiers in Cell and Developmental Biology·Carmen Valente, Antonino Colanzi
Feb 4, 2010·Molecular and Cellular Neurosciences·Leslayann C SchectersonMark Bothwell
Jun 11, 2009·Seminars in Cell & Developmental Biology·Jen-Hsuan Wei, Joachim Seemann
Aug 11, 2007·Current Opinion in Cell Biology·Antonino Colanzi, Daniela Corda
Sep 16, 2006·The EMBO Journal·Yunrui DuSusan Ferro-Novick
Oct 20, 2012·Traffic·Jorge Cancino, Alberto Luini
Nov 3, 2010·Traffic·Jen-Hsuan Wei, Joachim Seemann
Mar 11, 2015·Biology of the Cell·Hesso Farhan
Jan 18, 2011·The Biochemical Journal·Fabian P VinkeCatherine Rabouille
Apr 1, 2015·Future Medicinal Chemistry·Zutao YuQianbin Li
Nov 23, 2006·Biochimica Et Biophysica Acta·Yoav D Shaul, Rony Seger
Nov 10, 2009·Advances in Enzyme Regulation·Lisa PolzienUlf R Rapp
Dec 17, 2008·Molecular BioSystems·Anastasia Blagoveshchenskaya, Peter Mayinger
Jul 26, 2016·Frontiers in Cell and Developmental Biology·Marc K Saba-El-LeilSylvain Meloche
Oct 7, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Prajakta Gosavi, Paul A Gleeson
Jan 15, 2014·Molecular and Cellular Biology·Maria G Füzesi-LeviMichal Sharon

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
flow cytometry
immunoprecipitation
FACS
chemical treatments
transfection
FCS

Software Mentioned

view
CellQuest

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.