ERK5 pathway regulates the phosphorylation of tumour suppressor hDlg during mitosis.

Biochemical and Biophysical Research Communications
Francisco A Iñesta-VaqueraA Cuenda

Abstract

Human disc-large (hDlg) is a scaffold protein critical for the maintenance of cell polarity and adhesion. hDlg is thought to be a tumour suppressor that regulates the cell cycle and proliferation. However, the mechanism and pathways involved in hDlg regulation during these processes is still unclear. Here we report that hDlg is phosphorylated during mitosis, and we establish the identity of at least three residues phosphorylated in hDlg; some are previously unreported. Phosphorylation affects hDlg localisation excluding it from the contact point between the two daughter cells. Our results reveal a previously unreported pathway for hDlg phosphorylation in mitosis and show that ERK5 pathway mediates hDlg cell cycle dependent phosphorylation. This is likely to have important implications in the correct timely mitotic entry and mitosis progression.

References

Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·S S LeeR T Javier
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GaudetR A Lue
Nov 28, 2001·The Journal of Biological Chemistry·Margaret McLaughlinAlain Viel
Oct 22, 2003·Experimental Cell Research·Paola MassimiLawrence Banks
Mar 10, 2005·The Journal of Biological Chemistry·Yvonne KumaAna Cuenda
Mar 19, 2005·Science·Michael Glotzer
Jun 15, 2005·Annual Review of Biochemistry·Lars FunkeDavid S Bredt
Mar 3, 2006·The EMBO Journal·Kristopher K FreseRonald T Javier
Oct 21, 2006·Nature Immunology·Matthew F Krummel, Ian Macara
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zhen X MahoneyWojciech Swat
Apr 17, 2007·Development·Akiko Iizuka-KogoTakao Senda
Apr 25, 2007·The Journal of Cell Biology·Kelly CudeZhengui Xia
Jul 13, 2007·Cellular Signalling·Ana GírioSukalyan Chatterjee
Sep 14, 2007·The Biochemical Journal·Jenny BainPhilip Cohen
Sep 2, 2008·Experimental Cell Research·Kenji UnnoAthar H Chishti
Dec 11, 2008·Journal of Cell Science·Nisha NarayanLawrence Banks

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Citations

Aug 27, 2015·Science Signaling·Kevin WilhelmsenJudith Hellman
Jan 21, 2012·Biochemical Society Transactions·Pamela A LochheadSimon J Cook
Sep 20, 2011·Cellular Signalling·Rebecca GilleySimon J Cook
Jan 31, 2012·Chemistry & Biology·Tinghu ZhangNathanael S Gray
Feb 26, 2016·Journal of Receptor and Signal Transduction Research·Sudheer Kumar KatariAmineni Umamaheswari
Nov 21, 2020·Environmental Pollution·Francisco Inesta-VaqueraC Roland Wolf
Oct 17, 2021·Journal of Cellular and Molecular Medicine·Marta Ortega-MuelasRicardo Sánchez-Prieto

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