Ero1p: a novel and ubiquitous protein with an essential role in oxidative protein folding in the endoplasmic reticulum

Molecular Cell
M G PollardJ S Weissman

Abstract

The structure of many proteins entering the secretory pathway is dependent on stabilization by disulfide bonds. To support disulfide-linked folding, the endoplasmic reticulum (ER) must maintain a strongly oxidizing environment compared to the highly reduced environment of the cytosol. We report here the identification and characterization of Ero1p, a novel and essential ER-resident protein. Mutations in Ero1p cause extreme sensitivity to the reducing agent DTT, whereas overexpression confers DTT resistance. Strikingly, compromised Ero1p function results in ER retention of disulfide-stabilized proteins in a reduced, nonnative form, while not affecting structural maturation of a disulfide-free protein. We conclude that there exists a specific cellular redox machinery required for disulfide-linked protein folding in the ER and that Ero1p is an essential component of this machinery.

References

Jan 2, 1992·Gene·T W ChristiansonP Hieter
Jan 1, 1991·Methods in Enzymology·F Sherman
May 25, 1990·Nucleic Acids Research·M E SchmittB L Trumpower
Jan 1, 1989·Annual Review of Cell Biology·S M Hurtley, A Helenius
May 1, 1967·Journal of Bacteriology·L H Hartwell
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K A Nasmyth, S I Reed
Nov 1, 1995·The Journal of Cell Biology·K G Hardwick, A W Murray
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D MissiakasS Raina
Jul 1, 1995·The Journal of Cell Biology·J F SimonsA Helenius
Nov 1, 1994·The Biochemical Journal·T H FarmerD J Knowles
Feb 1, 1997·Trends in Biochemical Sciences·D Missiakas, S Raina
Apr 1, 1997·Journal of Bacteriology·D Missiakas, S Raina
Apr 1, 1997·Protein Science : a Publication of the Protein Society·W A Fenton, A L Horwich
Aug 1, 1992·Trends in Cell Biology·A HeleniusI Braakman

❮ Previous
Next ❯

Citations

Aug 5, 2003·BioFactors·Gábor BánhegyiJózsef Mandl
Sep 29, 2004·Histochemistry and Cell Biology·Leni Moldovan, Nicanor I Moldovan
Nov 18, 2005·Molecular Genetics and Genomics : MGG·Yukiko TakemoriHiroshi Sakurai
Oct 24, 2012·Protoplasma·Isabel Aller, Andreas J Meyer
Jun 15, 2007·Antonie van Leeuwenhoek·H Reynaldo López-Mirabal, Jakob R Winther
May 26, 2004·Biochimica Et Biophysica Acta·Bonney Wilkinson, Hiram F Gilbert
Apr 5, 2005·Methods : a Companion to Methods in Enzymology·Davis T W Ng
Aug 15, 2002·Archives of Biochemistry and Biophysics·Colin ThorpeDonald L Coppock
Jun 8, 2000·FEMS Microbiology Reviews·R A FabianekL Thöny-Meyer
Apr 8, 2000·Trends in Cell Biology·A R FrandC A Kaiser
Sep 28, 2000·Current Opinion in Chemical Biology·K J Woycechowsky, R T Raines
Feb 15, 2007·Biochemistry·Wenzhong WangColin Thorpe
Jul 25, 2009·Nature Reviews. Molecular Cell Biology·Ineke Braakman
Jun 29, 2004·Nature Structural & Molecular Biology·James C A Bardwell
Mar 23, 1999·The Biochemical Journal·D M Ferrari, H D Söling
May 20, 2003·European Journal of Biochemistry·Bernhard GessArmin Kurtz
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yayoi OndaYasushi Kawagoe
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·J K SuhJ D Robertus
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·J K Suh, J D Robertus
Nov 26, 2009·The Journal of Biological Chemistry·Valerie N Olmo, Eric Grote
Jan 9, 2009·The Journal of Biological Chemistry·Jon Beckwith
Sep 19, 2003·Antioxidants & Redox Signaling·Adam Benham
Sep 19, 2003·Antioxidants & Redox Signaling·David P DixonAdam Benham
Sep 19, 2003·Antioxidants & Redox Signaling·Elizabeth A Kersteen, Ronald T Raines
Sep 22, 2006·Antioxidants & Redox Signaling·Agnes GörlachThomas Kietzmann
May 9, 2006·Antioxidants & Redox Signaling·Adam M Benham, Roberto Sitia
May 9, 2006·Antioxidants & Redox Signaling·Donald L Coppock, Colin Thorpe
Jun 15, 2006·Antioxidants & Redox Signaling·Carolyn S Sevier, Chris A Kaiser
Nov 6, 2007·Antioxidants & Redox Signaling·Jyoti D Malhotra, Randal J Kaufman
Jan 31, 2009·Antioxidants & Redox Signaling·Youngkyun KimKwangseog Ahn
May 30, 2009·Antioxidants & Redox Signaling·Feras Hatahet, Lloyd W Ruddock
Feb 27, 2009·Antioxidants & Redox Signaling·Yuichiro Shimizu, Linda M Hendershot
Dec 17, 2009·Antioxidants & Redox Signaling·Miklós CsalaGábor Bánhegyi
Aug 19, 2009·Antioxidants & Redox Signaling·Mirva J SaaranenLloyd W Ruddock
Jan 26, 2010·Antioxidants & Redox Signaling·Balázs EnyediMiklós Geiszt
Mar 11, 2010·Antioxidants & Redox Signaling·Dionisia P Sideris, Kostas Tokatlidis
May 22, 2010·Antioxidants & Redox Signaling·Timothy J Tavender, Neil J Bulleid
Jan 19, 2011·Antioxidants & Redox Signaling·Stephen R ShouldiceJennifer L Martin
Dec 8, 2011·Antioxidants & Redox Signaling·Taichi KakihanaRoberto Sitia
Dec 8, 2011·Antioxidants & Redox Signaling·Kazutaka Araki, Kenji Inaba

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