EROD and MROD as Markers of Cytochrome P450 1A Activities in Hepatic Microsomes from Entire and Castrated Male Pigs.

Sensors
Galia Zamaratskaia, V Zlabek

Abstract

In the present study, we characterized the kinetic parameters of 7-ethoxy-resorufin O-deethylation (EROD) and 7-methoxyresorufin O-demethylation (MROD) in hepatic microsomes from entire and castrated male pigs. Validation parameters of an HPLC-based method to analyse EROD and MROD activities are also described. Eadie-Hofstee plot analysis demonstrated a biphasic kinetic of EROD, indicating that at least two forms of cytochrome P450 are involved in this reaction. MROD followed monophasic kinetic, suggesting that a single enzyme, or enzymes with similar affinities, is responsible for the reaction. Inhibitory effects of α-naphthoflavone (ANF), ellipticine and furafylline were studied using microsomes from entire and castrated male pigs. ANF is a known inhibitor of both cytochrome P(450) 1A1 and 1A2 (CYP1A1 and CYP1A2); the presence of ANF in the incubations resulted in the inhibition of both EROD and MROD activities in porcine liver microsomes. EROD activities in porcine liver microsomes were also inhibited by selective CYP1A1 inhibitor ellipticine, but not by CYP1A2 inhibitor furafylline. MROD activities were strongly inhibited by ellipticine and to a much lesser extent by furafylline. Further studies are needed to evaluate subst...Continue Reading

References

Jun 1, 1990·British Journal of Clinical Pharmacology·D SesardicD S Davies
Mar 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·N TakahashiG S Bailey
Aug 30, 1994·Biochemical Pharmacology·M D BurkeR T Mayer
Feb 21, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yune-Fang UengChieh-Fu Chen
Oct 24, 2003·Archives of Toxicology·Miroslav MachalaLenka Skálová
Sep 5, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Sompon Wanwimolruk, Preeya Wanwimolruk
Oct 25, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A MessinaV Longo
Dec 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M MonshouwerA S van Miert
Aug 1, 2009·Animal : an International Journal of Animal Bioscience·G ZamaratskaiaA Madej
Nov 2, 2007·Sensors·Marcela HavelkováZdeňka Svobodová

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Citations

May 12, 2011·Fish Physiology and Biochemistry·Galia Zamaratskaia, Vladimir Zlabek
Mar 23, 2012·Animal : an International Journal of Animal Bioscience·C BruniusG Zamaratskaia
Mar 23, 2012·Animal : an International Journal of Animal Bioscience·V Zlabek, G Zamaratskaia
Jul 15, 2011·Interdisciplinary Toxicology·Galia ZamaratskaiaVladimir Zlabek
Feb 6, 2016·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Rachel E PetersSteven D Siciliano
Jan 26, 2016·Journal of Agricultural and Food Chemistry·Christoph GerlachMatthias Wuest
Jan 17, 2012·Ecotoxicology and Environmental Safety·V BurkinaV Zlabek
Oct 14, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Martin Krøyer RasmussenGalia Zamaratskaia
Nov 26, 2009·Reproduction in Domestic Animals = Zuchthygiene·G ZamaratskaiaØ Andresen
Jun 11, 2015·Cell Biochemistry and Function·Anthony LynnBrian Ratcliffe
Nov 20, 2014·Computational and Structural Biotechnology Journal·Martin Krøyer Rasmussen, Galia Zamaratskaia
Apr 16, 2016·Animal : an International Journal of Animal Bioscience·C BruniusG Zamaratskaia
Jun 22, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Martin Krøyer RasmussenBo Ekstrand
Dec 4, 2014·Animal : an International Journal of Animal Bioscience·F Borrisser-PairóG Zamaratskaia
Oct 23, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Marilia N SiebertAfonso C D Bainy
Apr 6, 2016·Toxicologic Pathology·Kristi L HelkeCharlotte Hollinger
Feb 9, 2012·Molecular Biology Reports·AnnaLotta Schiller VestergrenGalia Zamaratskaia
Nov 22, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Konrad A SzychowskiJan Gmiński
Jan 12, 2021·Toxicology Letters·Viktoriia BurkinaMartin Krøyer Rasmussen
May 21, 2020·Journal of Agricultural and Food Chemistry·Jie ShiChunbao Li
Jul 9, 2021·European Journal of Drug Metabolism and Pharmacokinetics·Sherif M ShoiebAyman O S El-Kadi
Oct 27, 2020·Analytical Chemistry·Gowtham SathyanarayananTiina Sikanen

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Software Mentioned

GraphPad Prism
GraphPad
SigmaPlot Enzyme Kinetics
6000 HPLC Manager

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