Erroneous ribosomal RNAs promote the generation of antisense ribosomal siRNA

Proceedings of the National Academy of Sciences of the United States of America
Chengming ZhuShouhong Guang

Abstract

Ribosome biogenesis is a multistep process, during which mistakes can occur at any step of pre-rRNA processing, modification, and ribosome assembly. Misprocessed rRNAs are usually detected and degraded by surveillance machineries. Recently, we identified a class of antisense ribosomal siRNAs (risiRNAs) that down-regulate pre-rRNAs through the nuclear RNAi pathway. To further understand the biological roles of risiRNAs, we conducted both forward and reverse genetic screens to search for more suppressor of siRNA (susi) mutants. We isolated a number of genes that are broadly conserved from yeast to humans and are involved in pre-rRNA modification and processing. Among them, SUSI-2(ceRRP8) is homologous to human RRP8 and engages in m1A methylation of the 26S rRNA. C27F2.4(ceBUD23) is an m7G-methyltransferase of the 18S rRNA. E02H1.1(ceDIMT1L) is a predicted m6(2)Am6(2)A-methyltransferase of the 18S rRNA. Mutation of these genes led to a deficiency in modification of rRNAs and elicited accumulation of risiRNAs, which further triggered the cytoplasmic-to-nuclear and cytoplasmic-to-nucleolar translocations of the Argonaute protein NRDE-3. The rRNA processing deficiency also resulted in accumulation of risiRNAs. We also isolated SUSI-3...Continue Reading

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Citations

Mar 19, 2020·RNA Biology·Domenico RosaceSandra Blanco
Apr 27, 2019·Frontiers in Genetics·Qi YanXuezhu Feng
Oct 10, 2020·Science·Haijun WuZhong Zhao
Jan 10, 2021·International Journal of Molecular Sciences·Eleni G KaliatsiVassiliki Stamatopoulou
Aug 9, 2021·Nucleic Acids Research·Shimiao LiaoXuezhu Feng
Dec 23, 2021·Cell Death & Disease·Bowen LiuTianjiao Wang

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