PMID: 6166593Mar 1, 1981Paper

Errors in microdensitometry

The Histochemical Journal
D J Goldstein

Abstract

Microdensitometric errors can originate in the instrument, in the specimen or in the human operator. Instrumental sources of systematic error mostly reduce the apparent integrated absorbance, especially of relatively small and highly absorbing objects. They can be assessed, minimized or eliminated by available techniques, but with modern apparatus are in general important only if results of high accuracy are required. Instrument errors include: (a) distributional error, due to the use of too large a measuring spot or the specimen being out of focus; (b) glare (stray light), due mainly to multiple reflections in the microscope objective; (c) monochromator error (the use of insufficiently pure light); (d) calibration errors; and (e) errors resulting from lack of photometric linearity, or the specimen absorbance exceeding the measuring range of the instrument. Specimen errors, including the problems of specificity and stoichiometry, are now the most important obstacles to a wider use of microdensitometry. The following selected topics are briefly discussed: fading; rate of staining; Beer's law deviations and the microdensitometry of opaque particles. Human errors include faulty logic, and failing to attempt an investigation becaus...Continue Reading

References

Sep 1, 1975·Stain Technology·C D Dewse, C G Potter
Oct 1, 1976·The Journal of Cell Biology·K S Bedi, D J Goldstein
Nov 1, 1977·The Histochemical Journal·C L Scholtz
Nov 1, 1977·The Histochemical Journal·J P Mitchell
Dec 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M L Mello
Apr 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D G ChangarisJ W Combs
Jun 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A J StereA Anthony
Sep 1, 1975·Journal of Microscopy·D J Goldstein
Mar 1, 1976·The Histochemical Journal·T H Rosenquist
Jan 1, 1970·Journal of Microscopy·D J Goldstein
Jan 1, 1974·Chromosoma·N G Martin
Jan 1, 1966·Histochemie. Histochemistry. Histochimie·D Müller
Sep 1, 1974·British Journal of Haematology·V James, D J Goldstein
Jan 1, 1972·Histochemie. Histochemistry. Histochimie·K WürthnerJ Bahnsen
Jun 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B H Mayall, M L Mendelsohn
Jan 1, 1971·Chromosoma·E M RaschR W Rasch
Aug 1, 1980·Journal of Microscopy·D J Goldstein
May 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W A DuijndamA C Verweij
May 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W A DuijndamS H Riddersma
Jan 5, 1963·British Medical Journal·W J DEMPSTER, M A WILLIAMS
Aug 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J W KELLYH K HAWKINS
Jul 1, 1959·Physics in Medicine and Biology·K W KEOHANE, W K METCALF

❮ Previous
Next ❯

Citations

Aug 1, 1983·The Histochemical Journal·M L Mello
Sep 1, 1989·The Histochemical Journal·M Ruhnke, R Gossrau
Mar 13, 1998·Journal of Neuroscience Methods·O Guntinas-LichiusW F Neiss
Jul 1, 1986·The Histochemical Journal·C J Van Noorden, R G Butcher
Jun 1, 1983·The Histochemical Journal·C J Van NoordenI M Vogels
Nov 14, 2012·Progress in Histochemistry and Cytochemistry·Pasquale ChiecoCornelis J F Van Noorden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.