Er:YAG laser promotes proliferation and wound healing capacity of human periodontal ligament fibroblasts through Galectin-7 induction.

Journal of the Formosan Medical Association = Taiwan Yi Zhi
Taichen LinChun-Jung Chen

Abstract

Among various dental lasers, the erbium-doped yttrium-aluminum-garnet (Er:YAG) laser has great potential for periodontal treatment including soft and hard tissue ablation with minimal thermal side effects under suitable energy densities and it has multiple effects on tissues for wound-healing benefits. In the present study, we sought to reveal the molecular mechanism underlying the impact of Er:YAG laser on PDL fibroblasts. Cells were irradiated by a Er:YAG laser with various energy densities (3.6-6.3 J/cm2). MTT assay was used for cell proliferation, and the transwell system was employed for migration and invasion abilities. The wound healing capacity was evaluated by a scratch assay. After confirming these effects, qRT-PCR and western blotting analysis was applied to identify the differentially galectin-7 expression in the irradiated cells. Knockdown experiments were conducted to reveal the functional role of galectin-7 in the modulation of Er:YAG laser-mediated effects. 4.2 J/cm2 was the lowest energy density to induce the optimal cell proliferation, migration and invasion abilities. In the group of upregulated genes, galectin-7 was selected for further examination and its elevation after Er:YAG laser treatment was validated...Continue Reading

Citations

Dec 2, 2020·International Journal of Molecular Sciences·Yujin OhsugiTakanori Iwata
Aug 3, 2021·Photobiomodulation, Photomedicine, and Laser Surgery·Tsuyoshi ShimohiraAkira Aoki
Aug 29, 2021·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Chao-Yen HuangTaichen Lin

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