PMID: 9555101May 23, 1998Paper

Erythrocyte defenses against hydrogen peroxide: the role of ascorbic acid

Biochimica Et Biophysica Acta
S MendirattaJ M May

Abstract

Ascorbate has been reported to increase intracellular hydrogen peroxide (H2O2) generation in human erythrocytes. In the present work, the basis for this prooxidant effect of the vitamin was investigated in the context of erythrocyte defenses against H2O2. Ascorbate added to erythrocytes caused a dose-dependent increase in intracellular H2O2, which was measured as inactivation of endogenous catalase in the presence of 3-amino-1,2,4-triazole (aminotriazole). Ascorbate-induced catalase inactivation was not observed when only the intracellular ascorbate concentration was increased, when cells were incubated with ascorbate in plasma, or when extracellular Fe3+ was chelated. Together, these results suggest that the observed ascorbate-induced H2O2 generation is due to Fe3+-catalyzed oxidation of extracellular, as opposed to intracellular, ascorbate by molecular oxygen. Rather than generate an oxidant stress in erythrocytes, ascorbate was one of the most sensitive intracellular antioxidants to H2O2 coming from outside the cells. On the other hand, intracellular ascorbate contributed little to the detoxification of H2O2, which was found to be mediated by both catalase and by the GSH system.

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Citations

Jun 21, 2002·Life Sciences·Cheng-Gang ZouGraham Lloyd Jones
Aug 27, 2005·Mitochondrion·Xia Li, James M May
Apr 5, 2003·Applied and Environmental Microbiology·J B CrossP D Gladen
Jan 29, 2005·Blood·Nicos KarasavvasDavid W Golde
Mar 1, 2007·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Woo Suk KohChan H Park
Nov 18, 2010·Archives of Biochemistry and Biophysics·Isaac GinsburgErez Koren
Jan 26, 2010·Archives of Drug Information·Clement YedjouPaul Tchounwou
Nov 4, 2004·Respiratory Physiology & Neurobiology·Yves JammesStéphane Delliaux
Aug 5, 2016·The Indian Journal of Medical Research·Neelam Marwaha
Oct 22, 2013·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Felipe StiggerSimone Marcuzzo
Aug 17, 2006·Antioxidants & Redox Signaling·Argirios E TsantesNikolaos M Sitaras
Jan 12, 2019·Neurotoxicity Research·Musthafa Mohamed EssaMohammed Akbar
Jan 6, 2006·Acta Biochimica Et Biophysica Sinica·Shu-De LiCheng-Gang Zou
Feb 21, 2021·Antioxidants & Redox Signaling·Carola Muñoz-MontesinoCoralia I Rivas
Nov 1, 2001·Biochimica Et Biophysica Acta·J M MayJ D Morrow

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