Erythrocyte ghost--buffer partition coefficients of phenobarbital, pentobarbital, and thiopental support the pH-partition hypothesis

Canadian Journal of Physiology and Pharmacology
K Korten, K W Miller

Abstract

The partition coefficient (lambda) between red cell ghosts and buffer has been determined for three barbiturates over a range of pH. Experimental partition coefficients were linearly proportional to the calculated degree of association of the barbiturates. Lambda was 9.5 +/- 0.52 for phenobarbital, 12.7 +/- 0.91 for pentobarbital, and 27 +/- 4.9 for thiopental in their acid forms. Lambda for all three barbiturates in their anionic forms was zero. Our data support the assumption of the pH-partition hypothesis that the dependence of lambda on pH in biological membranes behaves essentially like that in organic solvents. However, the relative magnitudes of the erythrocyte partition coefficients correlate much more closely with the physiological permeability constants than do those of organic solvents, which tend to overestimate the differences between these compounds.

References

Nov 1, 1990·British Journal of Pharmacology·B A DodsonK W Miller
Sep 1, 1996·Anesthesia and Analgesia·T A ThurstonB P Mathew
Oct 1, 1981·British Journal of Clinical Pharmacology·E A Taylor, P Turner

Related Concepts

Diabutal
Ampholytes
Sodipental
Red Cell Ghost
Luminal
Erythrocytes
Hydrogen-Ion Concentration
In Vitro [Publication Type]

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