Erythrocyte membrane protein alteration in diabetics

East African Medical Journal
E O AdewoyeO O Olorunsogo

Abstract

To study the protein components of the erythrocyte membranes of diabetic Nigerians and to compare the results with the erythrocyte membrane protein components of normal healthy Nigerians. Laboratory based cross-sectional study. Department of Medicine, University College Hospital (UCH), Ibadan and Biomembrane Research Laboratory, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria. The study was carried out in patients with insulin-dependent diabetes mellitus--IDDM (Type 1 diabetes), non-insulin dependent diabetes mellitus--NIDDM (Type 2 diabetes) and healthy human volunteers (HHm), which served as controls. The subjects were aged 30-65 years. There were 12 subjects in each of the IDDM and NIDDM) and 18 subjects in the HHm group. Blood samples (20 ml per subject) were obtained from each subject and erythrocyte ghost membranes were isolated separately from each sample. Total erythrocyte membrane protein concentration of each sample was determined using bovine serum albumin (BSA) as standard. The protein components of the erythrocyte ghost membranes were determined using sodium dodecyl sulphate polyacrylamide gel electrophoresis. All values given are the mean +/- standard deviation (+/- SD) of th...Continue Reading

Citations

Jan 16, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ioannis K PetropoulosIssidora S Papassideri
Jul 1, 2008·Indian Journal of Clinical Biochemistry : IJCB·Gauri S Vahalkar, Vijaya A Haldankar
Nov 10, 2009·Ageing Research Reviews·Maria N Starodubtseva
Jan 27, 2012·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Prachi GuptaSurendra Kumar Bansal
Sep 26, 2013·Human & Experimental Toxicology·Km RamkumarP Gunasekaran

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