Abstract
Erythrocyte shape deformations are related to different important illnesses. In this paper, we focus on one of the most important: the Sickle cell disease. This disease causes the hardening or polymerization of the hemoglobin that contains the erythrocytes. The study of this process using digital images of peripheral blood smears can offer useful results in the clinical diagnosis of these illnesses. In particular, it would be very valuable to find a rapid and reproducible automatic classification method to quantify the number of deformed cells and so gauge the severity of the illness. In this paper, we show the good results obtained in the automatic classification of erythrocytes in normal cells, sickle cells, and cells with other deformations, when we use a set of functions based on integral-geometry methods, an active contour-based segmentation method, and a k-NN classification algorithm. Blood specimens were obtained from patients with Sickle cell disease. Seventeen peripheral blood smears were obtained for the study, and 45 images of different fields were obtained. A specialist selected the cells to use, determining those cells which were normal, elongated, and with other deformations present in the images. A process of aut...Continue Reading
References
Jan 11, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J W BacusF E Trobaugh
Jan 1, 1984·Monographs in Clinical Cytology·J W Bacus
Oct 1, 1994·Cytometry·L L WheelessL J Benjamin
Jan 1, 1996·Blood Cells, Molecules & Diseases·T AsakuraK Ohene-Frempong
May 29, 1999·Journal of Microscopy·H J Gundersen Nielsen J
Aug 29, 2006·Medical & Biological Engineering & Computing·Nicholas E RossAdriano G Dusé
Apr 1, 2008·BMC Cell Biology·Minh-Tam LePeter R Preiser
Jan 24, 2009·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Tobias BergenThorsten Zerfass
Jan 27, 2009·Journal of Biomedical Informatics·Gloria DíazEduardo Romero
Feb 14, 2009·PLoS Computational Biology·John M HigginsL Mahadevan
Jun 7, 2011·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Ran LiuBahram Javidi
Feb 1, 1982·IEEE Transactions on Pattern Analysis and Machine Intelligence·J Kittler, P A Devijver
Dec 21, 2012·Journal of Microscopy·D K DasA K Ray
Feb 2, 2013·Journal of Biomedical Optics·Faliu YiPierre Marquet