Erythrocyte vesiculation: a self-protective mechanism?

British Journal of Haematology
Frans L A WillekensGiel J C G M Bosman

Abstract

Previous studies demonstrated that 20% of haemoglobin is lost from circulating erythrocytes during their total lifespan by vesiculation. To study whether removal molecules other than membrane-bound haemoglobin were present in erythrocyte-derived vesicles, flow cytometry and immunoblot analysis were employed to examine the presence of phosphatidylserine (PS) and IgG, and senescent cell antigens respectively. It was demonstrated that 67% of glycophorin A-positive vesicles exposed PS, and that half of these vesicles also contained IgG. Immunoblot analysis revealed the presence of a breakdown product of band 3 that reacted with antibodies directed against senescent erythrocyte antigen-associated band 3 sequences. In contrast, only the oldest erythrocytes contained senescent cell antigens and IgG, and only 0.1% of erythrocytes, of all ages, exposed PS. It was concluded that vesiculation constitutes a mechanism for the removal of erythrocyte membrane patches containing removal molecules, thereby postponing the untimely elimination of otherwise healthy erythrocytes. Consequently, these same removal molecules mediate the rapid removal of erythrocyte-derived vesicles from the circulation.

References

Jan 1, 1978·Journal of Supramolecular Structure·M M Kay
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M M Kay
Apr 1, 1988·American Journal of Hematology·W H Reinhart, S Chien
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M M KayP S Bassel
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K Schlüter, D Drenckhahn
Apr 1, 1968·The Journal of Clinical Investigation·C F Reed
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M M Kay
Jan 1, 1994·European Journal of Haematology·F H BoschM R Halie
Jan 10, 2006·Annals of the New York Academy of Sciences·Marguerite Kay
Jan 18, 2006·Immunity·Falk Nimmerjahn, Jeffrey V Ravetch
Mar 15, 2006·Trends in Cell Biology·Yi WuRaymond B Birge

❮ Previous
Next ❯

Citations

Oct 29, 2010·Annals of Hematology·Johannes ThalerIngrid Pabinger
Sep 17, 2009·Glycoconjugate Journal·Sumanta BasuAbhijit Chakrabarti
Aug 21, 2012·Cell Biochemistry and Biophysics·Daiana M VotaDaniela C Vittori
Oct 3, 2012·Antioxidants & Redox Signaling·Joseph M Rifkind, Enika Nagababu
Oct 22, 2010·Current Opinion in Hematology·Jean-Daniel TissotGiorgia Canellini
May 14, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Christoph Stoll, Willem F Wolkers
Jun 27, 2013·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Olivier RubinJean-Daniel Tissot
Jul 9, 2014·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Hamdy A IbrahimRania A Abd Elazim
Feb 6, 2013·Nutrition & Diabetes·M J AlkhatatbehL F Lincz
Feb 9, 2010·Cellular and Molecular Life Sciences : CMLS·João Luiz M Wanderley, Marcello A Barcinski
Jan 8, 2016·Progress in Lipid Research·Mélanie CarquinDonatienne Tyteca
Jun 25, 2015·Liver International : Official Journal of the International Association for the Study of the Liver·Francesca LupoLucia De Franceschi
May 20, 2011·Blood Cells, Molecules & Diseases·Sumanta BasuAbhijit Chakrabarti
Nov 10, 2015·Proteomics. Clinical Applications·Julien DelobelNiels Lion
Jul 7, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Marco GirasoleSimona Amiconi
Jun 29, 2015·Colloids and Surfaces. B, Biointerfaces·V Vodyanoy
Nov 26, 2009·Journal of Proteomics·Niels LionJean-Daniel Tissot
Aug 8, 2009·Journal of Proteomics·G J C G M BosmanV M J Novotný
Jun 17, 2015·Cellular and Molecular Life Sciences : CMLS·Mélanie CarquinDonatienne Tyteca
Nov 10, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Michel PrudentNiels Lion
Aug 19, 2015·Redox Biology·S VoskouM Kleanthous
Jan 15, 2009·Transfusion Medicine·G J C G M BosmanV M J Novotný
Aug 22, 2013·Microscopy Research and Technique·Timothy MooreVitaly Vodyanoy
Dec 24, 2010·Vox Sanguinis·L van de Watering
Nov 2, 2012·American Journal of Hematology·Robert S FrancoRobert M Cohen
Nov 6, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michael C LarsonCheryl A Hillery
Jul 3, 2013·Journal of Veterinary Internal Medicine·J M HerringS A Smith
Mar 21, 2012·British Journal of Haematology·Mehrdad GhashghaeiniaGiel J C G M Bosman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.