PMID: 6165240Jan 1, 1980Paper

Erythrocytes as carriers of chemotherapeutic agents for targeting the reticuloendothelial system

American Journal of Hematology
W E LynchA Ghaffar

Abstract

The object of this work was to define a model using hypotonically loaded erythrocytes as a vehicle to target drugs to the reticuloendothelial system (RES). The optimum hemolytic event was found to occur at 100 mOsm/kg using a 0.5-min exposure at 0 degrees C. Approximately one third of the total volume of the cells could be replaced with hypotonic drug solutions under these conditions. Although cytosine-beta-D-arabinofuranoside, ara C, is membrane permeable and could not be entrapped in the erythrocytes, phosphorylation of this nucleoside antimetabolite enabled it to be loaded efficiently. Actinomycin D could be loaded and retained within the cells at 0 degrees C, but 90% of this loaded drug leaked out of the erythrocytes in 1 min at 37 degrees C. Actinomycin D-DNA complexes, however, could be loaded and retained for longer periods. In this case, 50% of the DNA-bound drug was retained in the cells for one hour at 37 degrees C. It was found that the glycopeptide antitumor antibiotic, bleomycin, could be entrapped and retained in the cells without appreciable leakage. It was possible to load a human therapeutic dose of this drug in 1-2 ml of packed cells. Furthermore, it was demonstrated that bleomycin entrapped in erythrocytes wa...Continue Reading

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Citations

Jan 1, 1985·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·J R DeLoach
Jan 1, 1983·Pharmacology & Therapeutics·G M Ihler
Mar 12, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Carmen Gutiérrez MillánJosé M Lanao
Jun 10, 2014·Expert Opinion on Drug Delivery·Manisha BhateriaRabi Sankar Bhatta
Jan 25, 2014·Expert Opinion on Drug Delivery·Abdolhossein ZarrinMehrdad Hamidi
Oct 29, 2005·Drug Delivery·Carmen Gutiérrez MillánMiguel Arévalo
Aug 5, 2008·Translational Research : the Journal of Laboratory and Clinical Medicine·Carmen Gutiérrez MillánJosé M Lanao
Dec 14, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elsa BrionesJosé M Lanao
Jan 1, 1985·Critical Reviews in Oncology/hematology·R J Sokol, S Hewitt
Feb 1, 1986·European Journal of Clinical Investigation·H G EichlerS Gasić
Jul 23, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Se-woon ChoeBrian S Sorg
May 20, 2015·ACS Biomaterials Science & Engineering·Yixue SuJian Yang
Apr 1, 2017·Therapeutic Delivery·Mariacristina Gagliardi
Oct 1, 1986·Medicinal Research Reviews·J R DeLoach
Jan 1, 1987·Annals of the New York Academy of Sciences·R KirshG Poste
Mar 22, 2020·Pharmaceutics·Larisa KolevaElena Sinauridze
Aug 1, 1991·Journal of Neurology, Neurosurgery, and Psychiatry·R B LiptonP H Wiernik
Jan 25, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Francesca PierigèMauro Magnani
Mar 17, 2021·European Journal of Pharmacology·Nur Najihah Izzati Mat RaniMohd Cairul Iqbal Mohd Amin

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