Erythroid cell growth and differentiation in vitro in the simulated microgravity environment of the NASA rotating wall vessel bioreactor

In Vitro Cellular & Developmental Biology. Animal
A J Sytkowski, K L Davis

Abstract

Prolonged exposure of humans and experimental animals to the altered gravitational conditions of space flight has adverse effects on the lymphoid and erythroid hematopoietic systems. Although some information is available regarding the cellular and molecular changes in lymphocytes exposed to microgravity, little is known about the erythroid cellular changes that may underlie the reduction in erythropoiesis and resultant anemia. We now report a reduction in erythroid growth and a profound inhibition of erythropoietin (Epo)-induced differentiation in a ground-based simulated microgravity model system. Rauscher murine erythroleukemia cells were grown either in tissue culture vessels at 1 x g or in the simulated microgravity environment of the NASA-designed rotating wall vessel (RWV) bioreactor. Logarithmic growth was observed under both conditions; however, the doubling time in simulated microgravity was only one-half of that seen at 1 x g. No difference in apoptosis was detected. Induction with Epo at the initiation of the culture resulted in differentiation of approximately 25% of the cells at 1 x g, consistent with our previous observations. In contrast, induction with Epo at the initiation of simulated microgravity resulted in...Continue Reading

References

Jan 1, 1991·Advances in Space Biology and Medicine·A Cogoli, F K Gmünder
Nov 1, 1991·Experimental Cell Research·R P de GrootW Kruijer
Dec 15, 1983·Experientia·A Tschopp, A Cogoli
Jul 13, 1984·Science·A CogoliP Fuchs-Bislin
Jul 1, 1995·Physiological Reviews·P F Davies
Jan 1, 1994·Advances in Space Biology and Medicine·P J RijkenS W de Laat
Feb 1, 1993·Proceedings of the Society for Experimental Biology and Medicine·T J GoodwinM P Moyer
Mar 1, 1993·Journal of Cellular Biochemistry·P J DukeD Montufar-Solis
Jul 1, 1996·Journal of Applied Physiology·C P AlfreyM H Pickett
Jul 1, 1996·Journal of Applied Physiology·Z AllebbanA T Ichiki
Oct 1, 1996·Medicine and Science in Sports and Exercise·C P AlfreyT Driscoll
Dec 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A SchmittL Schaffar
May 10, 1997·Lancet·C P AlfreyT B Driscoll
May 1, 1997·In Vitro Cellular & Developmental Biology. Animal·R E AkinsC R Hartzell
May 1, 1997·In Vitro Cellular & Developmental Biology. Animal·J M JessupG Spaulding
May 1, 1997·In Vitro Cellular & Developmental Biology. Animal·T L Baker, T J Goodwin
May 1, 1997·In Vitro Cellular & Developmental Biology. Animal·T J GoodwinJ L Becker
May 1, 1997·In Vitro Cellular & Developmental Biology. Animal·G MolnarC R Hartzell
Jun 1, 1997·In Vitro Cellular & Developmental Biology. Animal·V ChopraE V Hannigan
Nov 14, 1997·AIDS Research and Human Retroviruses·L B MargolisJ Zimmerberg
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S GudiJ A Frangos
May 20, 1998·In Vitro Cellular & Developmental Biology. Animal·P I LelkesB R Unsworth
Aug 26, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M L LewisE H Piepmeier
Apr 13, 1999·Medical & Biological Engineering & Computing·C GranetM H Lafage-Proust
Jan 1, 1992·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·P J RijkenJ Boonstra

❮ Previous
Next ❯

Citations

Sep 1, 2005·European Journal of Clinical Investigation·C-L HsiehS-H Fang
May 27, 2004·Biotechnology and Bioengineering·Martin H RhielDavid W Murhammer
Sep 21, 2006·Biotechnology and Bioengineering·Yoshimi OhyabuToshimasa Uemura
Jan 11, 2014·Biotechnology Journal·Guillaume F RousseauLuc Douay
Aug 3, 2004·Journal of Clinical Pharmacology·Annemarie GraebeHartmut Derendorf
Apr 23, 2014·Journal of Tissue Engineering and Regenerative Medicine·Theun van Veen, John A Hunt
Jun 19, 2008·Genomics, Proteomics & Bioinformatics·Jade Q ClementBobby L Wilson
Jan 27, 2011·Acta Biochimica Et Biophysica Sinica·Rui MengPeng Shang
Apr 2, 2005·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·Kun XuArthur J Sytkowski
Jun 15, 2011·Molecular Biology Reports·Ping LiChang Yong Wang
Apr 6, 2007·Medical Hypotheses·Michael W C PayneGuy Trudel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis