Erythroid colony growth in congenital hypoplastic anemia

The Journal of Clinical Investigation
M H FreedmanE F Saunders


Four children with congenital hypoplastic anemia (Diamond-Blackfan syndrome) and 30 control children with normal erythropoiesis were studied by a cell culture method in which human marrow, grown in a plasma clot, responds to added erythropoietin (EPO) with the appearance of discrete colonies of nucleated erythroid cells. The colonies arise from EPO-responsive stem cells and are not related to the number of morphologically identifiable marrow erythroids plated. Results of studies on control marrow indicated that without EPO there was little or no colony formation. Increasing EPO doses or nucleated marrow cells per culture resulted in a linear increase in colony numbers. The optimal EPO concentration of 2.5 U/ml yielded a mean of 158 +/- 79 colonies/1 x 10(5) nucleated cells on day 7 of incubation. Even in the absence of recognizable erythroids, marrows of all four patients with anemia grew erythroid colonies. Two patients on no therapy had decreased colony numbers. The other two, on prednisone, had normal numbers. Sera from patients did not inhibit colony formation from either autologous or control marrow. In contrast, serum from an adult with acquired pure red cell aplasia produced striking inhibition of colony growth. It appea...Continue Reading


Jan 1, 1979·American Journal of Hematology·M H FreedmanE F Saunders
Jul 1, 1993·Stem Cells·A P GillioR J O'Reilly
Nov 25, 2000·British Journal of Haematology·C SieffD G Nathan
Mar 28, 2001·Ryōikibetsu shōkōgun shirīzu·M Teramura
May 11, 1978·The New England Journal of Medicine·D G NathanD E Housman
Oct 18, 1979·The New England Journal of Medicine·P Quesenberry, L Levitt
Jun 12, 1980·The New England Journal of Medicine·W B ErshlerN T Shahidi
Dec 15, 1983·The New England Journal of Medicine·B E GladerL K Diamond
Feb 1, 1978·The Journal of Experimental Medicine·D G NathanD E Housman
Mar 4, 2000·Current Opinion in Hematology·T N WilligC Sieff
Oct 1, 1978·British Journal of Haematology·E F Saunders, M H Freedman
Jun 1, 1994·British Journal of Haematology·N CasadevallL Coulombel
Mar 1, 1995·British Journal of Haematology·Colin P McGuckinE C Gordon-Smith
Nov 18, 1997·Acta Paediatrica Japonica; Overseas Edition·I SekineS Yoshioka
Feb 1, 1978·The Journal of Clinical Investigation·D G NathanD E Housman
Oct 7, 2000·Paediatric Drugs·I DianzaniUgo Ramenghi
Nov 19, 2013·Pediatric Clinics of North America·S Deborah Chirnomas, Gary M Kupfer
Mar 26, 2011·Seminars in Hematology·Anupama NarlaD G Nathan
Jul 27, 2010·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·Lydie Da CostaT Leblanc
Mar 31, 2009·Hematology/oncology Clinics of North America·Jeffrey M Lipton, Steven Ellis
Jan 27, 2009·Seminars in Perinatology·Angela Rivers, William B Slayton
Sep 24, 2005·Pediatric Transplantation·Douglas M SilversteinV Matti Vehaskari
Nov 18, 2003·British Journal of Haematology·William C Mentzer
Sep 1, 2006·British Journal of Haematology·Hanna T Gazda, C Sieff
Aug 2, 2008·British Journal of Haematology·Adrianna VlachosParticipants of Sixth Annual Daniella Maria Arturi International Consensus Conference
Dec 21, 2012·British Journal of Haematology·John H FargoB E Glader
Dec 5, 2009·British Journal of Haematology·C SieffHarvey F Lodish
Aug 1, 1995·The Central African Journal of Medicine·P Jacobs
Sep 1, 1989·British Journal of Haematology·P H TsaiJ M Lipton
Feb 1, 1981·British Journal of Haematology·M Y Gordon, E C Gordon-Smith
Jan 1, 1984·Critical Reviews in Oncology/hematology·H E Broxmeyer
Jan 27, 1998·Hematology/oncology Clinics of North America·O I Krijanovski, C Sieff
Jul 28, 2004·Pediatric Clinics of North America·Matthew J BizzarroRichard A Ehrenkranz
Sep 26, 2015·Seminars in Hematology·Kazusa Ishii, Neal S Young
Oct 13, 2000·Baillière's Best Practice & Research. Clinical Haematology·M H Freedman
Oct 11, 2014·Cell·Michael BuszczakSean J Morrison
Aug 24, 2016·Human Gene Therapy·Diane L D'Allard, Johnson M Liu
Apr 30, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Isao HamaguchiStefan Karlsson
Dec 6, 2018·British Journal of Haematology·Marije Bartels, Marc Bierings
Apr 1, 1979·Archives of Disease in Childhood·B P Alter, D G Nathan
Sep 1, 1991·Journal of Clinical Pathology·K P Schofield, D I Evans
Jan 1, 1981·American Journal of Medical Genetics·R E HarrisM E Hodes
Jan 1, 1983·European Journal of Clinical Pharmacology·J SäweA Rane
Jan 1, 1982·Acta Anaesthesiologica Scandinavica. Supplementum·A RaneL Kager
Mar 29, 2014·Expert Review of Hematology·Adrianna VlachosJeffrey M Lipton
Jul 10, 2001·British Journal of Haematology·A A ThompsonN C Schanen
Aug 1, 1988·Journal of Medical Genetics·Q Qazi, E G Kassner
Feb 13, 2001·British Journal of Haematology·P FischH E Schaefer
Jul 1, 1983·British Journal of Haematology·C Sieff
Sep 1, 1987·Annals of Tropical Paediatrics·M M LubaniA A Hassanein
Aug 1, 1985·Scandinavian Journal of Haematology·K SawadaT Konno

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Hypoplastic Anemia
Bone Marrow Cells
Cell Differentiation Process
Abnormal Red Blood Cell
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