PMID: 25744828Mar 7, 2015Paper

Erythromycin inhibits elastin peptides-induced differentiation of CD4⁺T cells into Th17 cells

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Jifeng LiuJianquan Zhang

Abstract

To investigate the effect of erythromycin on differentiation into T helper 17 (Th17) cells from CD4⁺T cells exposed to elastin peptides. The CD4⁺T cells from spleen of male BALB/c mice by magnetic bead sorting were randomly divided into the control group (group A), the elastin peptides group (group B) and erythromycin group(group C). The cells in group B and C were cultured in serum-free medium supplemented with 30 μg/mL elastin peptides. The cells in group C were additionally administrated with erythromycin at a dose of 100 μg/mL. All of the CD4⁺T cells were cultured in serum-free culture solution for 24 hours. The number of the Th17 cells was detected by flow cytometry and the expression of retinoic acid related orphan nuclear receptor γt (RORγt) mRNA was measured by fluorescence quantitative PCR in each group. The expressions of NF-κB(p65) and signal transducer and activator of transcription 3 (STAT3) were analyzed by Western blotting. The Th17 cells in group B [(11.32 ± 2.34)%] increased as compared with that in group A [(5.21 ± 1.36)%], and the expression of RORγt mRNA in group B was higher than that in group A. Furthermore, the expressions of NF-κB(p65) and STAT3 proteins in group B increased significantly as compared wit...Continue Reading

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