Erythromycin-resistant lactic acid bacteria in the healthy gut of vegans, ovo-lacto vegetarians and omnivores

PloS One
Vesna MilanovićFrancesca Clementi

Abstract

Diet can affect the diversity and composition of gut microbiota. Usage of antibiotics in food production and in human or veterinary medicine has resulted in the emergence of commensal antibiotic resistant bacteria in the human gut. The incidence of erythromycin-resistant lactic acid bacteria (LAB) in the feces of healthy vegans, ovo-lacto vegetarians and omnivores was analyzed. Overall, 155 LAB were isolated and characterized for their phenotypic and genotypic resistance to erythromycin. The isolates belonged to 11 different species within the Enterococcus and Streptococcus genera. Enterococcus faecium was the dominant species in isolates from all the dietary categories. Only 97 out of 155 isolates were resistant to erythromycin after Minimum Inhibitory Concentration (MIC) determination; among them, 19 isolates (7 from vegans, 4 from ovo-lacto vegetarians and 8 from omnivores) carried the erm(B) gene. The copresence of erm(B) and erm(A) genes was only observed in Enterococcus avium from omnivores. Moreover, the transferability of erythromycin resistance genes using multidrug-resistant (MDR) cultures selected from the three groups was assessed, and four out of six isolates were able to transfer the erm(B) gene. Overall, isolates...Continue Reading

References

Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 1, 1989·Clinical Microbiology Reviews·A L Coykendall
Jun 1, 1995·Trends in Microbiology·D B ClewellD D Jaworski
Jul 12, 1997·Lancet·N van den BraakH Endtz
Mar 18, 2000·Antimicrobial Agents and Chemotherapy·A PortilloC Torres
Jan 18, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Roland Leclercq
Sep 19, 2003·Annals of Internal Medicine·Maria SjölundLars Engstrand
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Jan 18, 2006·International Journal of Food Microbiology·Sigrid R AndersenJeppe Boel
Sep 15, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R Pérez-PulidoA Gálvez
Sep 29, 2006·International Journal of Food Microbiology·Cristiana GarofaloFrancesca Biavasco
Feb 6, 2008·Journal of Basic Microbiology·Gustavo Pelicioli RiboldiJeverson Frazzon
Jun 11, 2008·Food Microbiology·Bruna C GomesElaine C P De Martinis
Jul 29, 2010·Physiological Reviews·Inna SekirovB Brett Finlay
Jul 31, 2010·Journal of Medical Microbiology·Jannine K BaileyRuth M Hall
Aug 14, 2010·Microbiology·Cecilia JernbergJanet K Jansson
Nov 15, 2011·Current Opinion in Gastroenterology·Vincent B Young
Jun 28, 2013·Frontiers in Microbiology·Jean-Marc Rolain
Jul 3, 2013·International Journal of Environmental Research and Public Health·Claire VerraesLieve Herman
Oct 12, 2013·Frontiers in Microbiology·Chiara DevirgiliisGiuditta Perozzi
Nov 1, 2013·European Journal of Nutrition·Bojana Bogovič MatijašićIrena Rogelj
Dec 18, 2013·Nature·Lawrence A DavidPeter J Turnbaugh
Dec 18, 2013·The Journal of Antimicrobial Chemotherapy·Andrea BrencianiEleonora Giovanetti
Oct 1, 2014·The Proceedings of the Nutrition Society·Harry J FlintPetra Louis
Dec 2, 2014·International Journal of Food Microbiology·Andrea OsimaniFrancesca Clementi
Dec 30, 2014·International Journal of Food Microbiology·Sivaranjani PagadalaRobert L Buchanan
Jan 27, 2015·Frontiers in Microbiology·Henk Aarts, Abelardo Margolles
Apr 17, 2015·Infection and Drug Resistance·Vangelis Economou, Panagiota Gousia
Apr 29, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Willem van Schaik

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Basic local Alignment Search Tool ( BLAST )
BLAST

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