Erythropoietin alleviates hyperglycaemia-associated inflammation by regulating macrophage polarization via the JAK2/STAT3 signalling pathway

Molecular Immunology
Jinjin CuiBo Yu

Abstract

Erythropoietin (EPO), which is clinically used for renal anaemia, reportedly exerts beneficial pleiotropic effects in atherosclerosis. This aim of this study was to investigate the effects of EPO on macrophage inflammation and polarization under hyperglycaemic conditions and to identify the effects of EPO-treated macrophage supernatants (SNs) on endothelial cell (EC) function. Peritoneal macrophages (pMΦs) were isolated from normal, diabetic or EPO-injected mice. Pro-inflammatory factors were detected by qRT-PCR and ELISA, and macrophage phenotype markers were evaluated by flow cytometry. High glucose culture was used to mimic the hyperglycaemic microenvironment of diabetes mellitus (DM) in vitro. After exposure to various doses of stimuli, macrophage inflammation and phenotype were detected via ELISA, qRT-PCR and flow cytometry. The underlying mechanism was investigated through western blotting. To examine the communication between macrophages and ECs, ECs were cultured with the SN of macrophages treated with different stimuli, and the tube formation ability of ECs was detected using Matrigel. The VEGF, ICAM-1 and VCAM-1 protein expression levels were determined by western blotting, and the nitric oxide (NO) and endothelin-1 (...Continue Reading

Citations

Jul 10, 2019·PloS One·Daniela FrascaBonnie B Blomberg
Mar 15, 2020·BMC Cardiovascular Disorders·Xilan YangChen Qu
Dec 26, 2018·Journal of Cellular Physiology·Xiaowen ChiWei Zhang
Dec 16, 2019·Biochemical and Biophysical Research Communications·Ji-Ying ChenYuan Guo
Apr 4, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhongnan YangFengmin Shao
Jun 29, 2021·Journal of Biomaterials Applications·Lisheng ZhuGang Peng

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