Erythropoietin and erythropoietin receptor in the developing human central nervous system

Pediatric Research
S E JuulR D Christensen

Abstract

We have previously shown the presence of erythropoietin (Epo) within the spinal fluid of normal preterm and term infants, and the presence of Epo receptor (Epo-R) in the spinal cords of human fetuses. It is not known, however: 1) whether cells within the fetal central nervous system (CNS) express Epo; 2) if so, whether this expression changes with development; 3) which cells within the CNS express Epo-R; 4) whether Epo-R expression within the CNS changes with development; and 5) whether Epo-R within the fetal CNS are functional. Expression of mRNA for Epo and Epo-R was sought by reverse transcription-PCR in mixed primary cultures of fetal spinal cords as well as NT2 and hNT cells, human cell lines of neuronal precursors and mature neurons, respectively. Epo was measured by ELISA in spent media from primary cell culture, and immunohistochemistry was used to identify Epo-R on neurons and glia in cell culture, and in brain sections. Developmental changes in Epo and Epo-R expression were sought in spinal cords and brains from fetuses of 7-24 wk postconception by semiquantitative PCR. To assess Epo-R function, NT2 cells were exposed to conditions which stimulate programmed cell death, and rescue from apoptosis by the addition of rec...Continue Reading

References

Oct 1, 1992·The Journal of Comparative Neurology·J F GadisseuxP Evrard
Jan 1, 1991·The American Journal of Pediatric Hematology/oncology·J L AscensaoE D Zanjani
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A AnagnostouM Steiner
Oct 1, 1985·Pathology·S E FrancisH Kronenberg
Jan 1, 1989·Advances in Experimental Medicine and Biology·N Beru, E Goldwasser
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Nakajima-IijimaT Kakunaga
Aug 1, 1984·The Anatomical Record·E KelemenT M Fliedner
Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·T TabiraF Gallyas
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M CarrollA D D'Andrea
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·M DigicayliogluM Gassmann
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·P G FarlieO Bernard
Nov 1, 1994·Developmental Biology·Z Y LiuC T Noguchi
Jan 1, 1994·Blood Purification·A R Nissenson
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·A AnagnostouC T Noguchi
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L T ZhongD E Bredesen
Jun 1, 1996·Journal of Cellular Physiology·M R WaldL Sterin-Borda
Jul 15, 1996·European Journal of Biochemistry·R YamajiY Nakano
Mar 1, 1997·The Journal of Pediatrics·S E JuulR D Christensen

❮ Previous
Next ❯

Citations

Dec 2, 2008·Expert Reviews in Molecular Medicine·Constance Tom NoguchiYi Jia
Feb 9, 2002·British Journal of Pharmacology·Jacob Bertram SpringborgNiels Vidiendal Olsen
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Murat CelikMichael Brines
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A L SirénP Ghezzi
Mar 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Laura CalvilloMichael Brines
May 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Serhat ErbayraktarMichael Brines
Jun 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Alfredo GorioMichael Brines
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·M L BrinesA Cerami
Jun 24, 2004·Archives of Disease in Childhood. Fetal and Neonatal Edition·T StrunkC Schultz
Aug 13, 2009·Developmental Neuroscience·Fernando F GonzalezDonna M Ferriero
Feb 9, 2013·Stroke; a Journal of Cerebral Circulation·Fernando F GonzalezDonna M Ferriero
Nov 1, 2008·Therapeutic Advances in Neurological Disorders·Claudia BartelsHannelore Ehrenreich
Feb 10, 2010·Experimental & Translational Stroke Medicine·Nadiya Byts, Anna-Leena Sirén
Jun 21, 2008·International Journal of Biological Sciences·Nikolaos PaschosAlexandros E Beris
Jan 16, 2020·The New England Journal of Medicine·Sandra E JuulUNKNOWN PENUT Trial Consortium
Dec 14, 2004·The Oncologist·Francis Farrell, Adrian Lee
Sep 25, 2002·The European Journal of Neuroscience·Mauro VairanoPierluigi Navarra
Sep 27, 2003·European Journal of Clinical Investigation·G J CaineG Y H Lip
Sep 27, 2003·European Journal of Clinical Investigation·D RibattiM Presta
Sep 27, 2003·Neuroreport·Hiroko MatsushitaMary Ann Wilson
May 15, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Joachim Fandrey
Aug 16, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·I YatsivE Shohami
Feb 19, 2014·Current Opinion in Pediatrics·Amber M Messier, Robin K Ohls
Apr 7, 2007·Journal of Neurosurgery·Ahmed MohyeldinAjay Verma
Aug 2, 2002·Nature Medicine·Steven A Goldman, Maiken Nedergaard
Mar 3, 2005·Nephron. Experimental Nephrology·Masayo H EchigoyaSatoshi Sasaki
May 24, 2006·Journal of Pharmacological Sciences·Daisuke NishiyaMinoru Yoshiyama
May 10, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·G ShenoudaT Chow
Feb 14, 2009·Cell and Tissue Research·Kathryn MunroMary Tolcos
Dec 27, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Anna-Leena SirénHannelore Ehrenreich
May 9, 2012·Anatomy Research International·Mawadda AlnaeeliConstance Tom Noguchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis