Erythropoietin (EPO) immunoaffinity columns--a powerful tool for purifying EPO and its recombinant analogues

Journal of Pharmaceutical and Biomedical Analysis
Yvette DehnesMaria Lönnberg

Abstract

The sample preparation method preceding the urinary erythropoietin (EPO) doping test is based on several concentration and ultrafiltration steps. In order to improve the quality of isoelectric focusing (IEF) gel results and therefore, the sensitivity of the EPO test, new sample preparation methods relying on affinity purification were recently proposed. This article focuses on the evaluation and validation of disposable immunoaffinity columns targeting both endogenous and recombinant EPO molecules in two World Anti-Doping Agency (WADA) accredited anti-doping laboratories. The use of the columns improved the resolution of the IEF profiles considerably when compared with the classical ultrafiltration method, and the columns' ability to ensure the isoform integrity of the endogenous and exogenous EPO molecules was confirmed. Immunoaffinity columns constitute therefore a potent and reliable tool for the preparation of urine samples and their use will significantly improve the sensitivity and specificity of the actual urinary EPO test.

References

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Jul 4, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Werner W Franke, Hans Heid
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Citations

Jun 4, 2011·Analytical and Bioanalytical Chemistry·Christian Reichel
Apr 25, 2012·Analytical and Bioanalytical Chemistry·Maria LönnbergLudovic Bailly-Chouriberry
May 10, 2013·Analytical and Bioanalytical Chemistry·M Lönnberg, C Lundby
Aug 10, 2011·Forensic Science International : Synergy·Flavia BadoudJean-Luc Veuthey
Jan 31, 2012·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Oct 19, 2011·Analytical Biochemistry·Maria LönnbergJan Carlsson
Nov 2, 2019·Drug Testing and Analysis·Xinmiao ZhouChunji He

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