Abstract
We have reported that erythropoietin induces a dose-dependent increase in cytosolic calcium ([Cai]) in single human peripheral blood BFU-E derived erythroblasts which is specific for stage of differentiation and that this increase is modulated by erythropoietin through an ion channel permeable to Ca2+. Here, the role of protein phosphorylation in the increase in intracellular free calcium [Cai] stimulated by erythropoietin was studied with digital video imaging. Preincubation of day 10 erythroblasts with a broad inhibitor of serine/threonine and tyrosine kinases, staurosporine (100 nM), blocked the increase in [Cai] over 20 min following erythropoietin stimulation. However, erythropoietin-induced calcium influx was unaffected by preincubation of cells with specific inhibitions of protein kinase C (calphostin C) or the cAMP- or cGMP-dependent kinases (KT 5720, HA 1004), and [Cai] did not increase following stimulation with phorbol 12-myristate 13-acetate (PMA) or dibutyryl cAMP. These results suggest that neither protein kinase C nor protein kinase A mediate the erythropoietin-induced [Cai] increase. In contrast, preincubation with genistein, a tyrosine kinase inhibitor, blocked the erythropoietin induced increase in [Cai]. To f...Continue Reading
References
Feb 1, 1992·Molecular and Cellular Biology·A Yoshimura, H F Lodish
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D LinnekinW L Farrar
Aug 14, 1992·Biochemical and Biophysical Research Communications·B L SlomianyA Slomiany
Sep 1, 1992·General Pharmacology·B L SlomianyA Slomiany
Nov 11, 1992·The Journal of Clinical Investigation·J Y CheungB A Miller
Oct 1, 1991·Molecular and Cellular Biology·O MiuraJ N Ihle
Jan 1, 1990·European Journal of Immunology·H G DrexlerA V Hoffbrand
Apr 15, 1991·Biochemical and Biophysical Research Communications·R F BrunsH Nakano
Jan 1, 1991·Biology of the Neonate·C BillatB Haye
May 31, 1991·Biochemical and Biophysical Research Communications·D I Yule, J A Williams
Sep 1, 1990·The Journal of Clinical Investigation·A D D'Andrea, L I Zon
Jun 1, 1990·Cell·A YataniA M Brown
Jun 1, 1990·The Journal of Clinical Investigation·R V YelamartyJ Y Cheung
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Apr 21, 1989·Cell·A D D'AndreaG G Wong
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K NunokiW A Catterall
Jun 1, 1989·Trends in Pharmacological Sciences·U T Rüegg, G M Burgess
Nov 1, 1988·Journal of Neurochemistry·H ShuntohC Tanaka
Dec 30, 1988·Biochemical and Biophysical Research Communications·Y Fujita-Yamaguchi, S Kathuria
Jan 30, 1987·Biochemical and Biophysical Research Communications·H KaseM Kaneko
Mar 13, 1986·Biochemical and Biophysical Research Communications·T TamaokiF Tomita
Apr 1, 1985·Cell Calcium·R Y TsienM Poenie
Oct 9, 1984·Biochemistry·H HidakaY Sasaki
Jul 30, 1993·Cell·B A WitthuhnJ N Ihle
May 7, 1993·Science·E GulbinsA Altman
Citations
Sep 6, 2000·Journal of Cellular Biochemistry·P T PhamC J Lynch
May 9, 1998·Kidney International·M B MarreroD C Eaton
May 3, 2000·Kidney International·D BanerjeeJ W Adamson
Oct 31, 2000·Bioscience, Biotechnology, and Biochemistry·R SasakiM Nagao
Feb 24, 2001·Journal of Hypertension·T AkimotoY Asano
Aug 1, 1996·Kidney International·H HallerF C Luft
Oct 13, 2010·Archives of Biochemistry and Biophysics·Mariana A CalleroAlcira B Nesse
May 28, 2016·Acta Physiologica·Y-B YuT-S Lee
Dec 31, 1997·The Journal of Biological Chemistry·G BabniggM L Villereal
Oct 17, 1998·The Journal of Biological Chemistry·B SorinP Vacher
Jul 10, 1999·The Journal of Biological Chemistry·B A MillerJ Y Cheung
Mar 18, 2003·Journal of Neuropathology and Experimental Neurology·M BuemiN Frisina
Feb 8, 2000·The Journal of Biological Chemistry·J M MasonD L Barber