PMID: 7536133Dec 1, 1994Paper

Erythropoietin modulation of intracellular calcium: a role for tyrosine phosphorylation

Cell Calcium
B A MillerJ Y Cheung

Abstract

We have reported that erythropoietin induces a dose-dependent increase in cytosolic calcium ([Cai]) in single human peripheral blood BFU-E derived erythroblasts which is specific for stage of differentiation and that this increase is modulated by erythropoietin through an ion channel permeable to Ca2+. Here, the role of protein phosphorylation in the increase in intracellular free calcium [Cai] stimulated by erythropoietin was studied with digital video imaging. Preincubation of day 10 erythroblasts with a broad inhibitor of serine/threonine and tyrosine kinases, staurosporine (100 nM), blocked the increase in [Cai] over 20 min following erythropoietin stimulation. However, erythropoietin-induced calcium influx was unaffected by preincubation of cells with specific inhibitions of protein kinase C (calphostin C) or the cAMP- or cGMP-dependent kinases (KT 5720, HA 1004), and [Cai] did not increase following stimulation with phorbol 12-myristate 13-acetate (PMA) or dibutyryl cAMP. These results suggest that neither protein kinase C nor protein kinase A mediate the erythropoietin-induced [Cai] increase. In contrast, preincubation with genistein, a tyrosine kinase inhibitor, blocked the erythropoietin induced increase in [Cai]. To f...Continue Reading

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Citations

Oct 31, 2000·Bioscience, Biotechnology, and Biochemistry·R SasakiM Nagao
Aug 1, 1996·Kidney International·H HallerF C Luft
Oct 13, 2010·Archives of Biochemistry and Biophysics·Mariana A CalleroAlcira B Nesse
Dec 31, 1997·The Journal of Biological Chemistry·G BabniggM L Villereal
Jul 10, 1999·The Journal of Biological Chemistry·B A MillerJ Y Cheung
Mar 18, 2003·Journal of Neuropathology and Experimental Neurology·M BuemiN Frisina

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