Erythropoietin mRNA expression in pig embryos

Animal Reproduction Science
H G KlemckeP L Pearson

Abstract

To address whether altered erythropoietin (EPO) synthesis might be involved in prenatal pig mortality, studies were conducted to measure porcine embryonic EPO mRNA expression during early gestation (days 24-40). Three pig models differing in embryonic survival from days 24-40 were investigated: intact white crossbred gilts (INT), white crossbred gilts that were unilaterally hysterectomized-ovariectomized before puberty and whose pregnant uterus constituted a crowded environment (UHO), and prolific, intact Meishan gilts (ME). A partial cDNA for porcine EPO, developed via reverse transcription and polymerase chain reaction procedures was used to generate a 32P-labeled probe for use in Northern analyses. In an initial study, embryonic liver EPO mRNA was greatest on day 24, decreased by day 30 (P<0.01), and was barely detectable by day 40. EPO mRNA expression was not influenced by pig model. Placental EPO mRNA expression was detectable in only 4 of 53 placentae examined. In a second study at day 35 of gestation, embryonic liver EPO mRNA expression was measured in the same three pig models and in two embryos of divergent weights from each gilt. Meishan embryos had lower (P<0.01) plasma immunoassayable EPO concentrations (P=0.04) and...Continue Reading

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Citations

Mar 26, 2002·Molecular and Cellular Endocrinology·R Bruce DavidE Marelyn Wintour
Nov 30, 2002·Domestic Animal Endocrinology·J G KimR K Christenson
Jan 25, 2006·Experimental Physiology·H G KlemckeR K Christenson
Sep 29, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Longchao ZhangLixian Wang
Oct 30, 2003·The Journal of Biological Chemistry·Christof DameJörg Bungert
Feb 16, 2006·Reproduction, Fertility, and Development·James C Cross

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