Erythropoietin promotes network formation of transplanted adipose tissue-derived microvascular fragments

European Cells & Materials
Philipp KarschniaMatthias W Laschke

Abstract

The seeding of tissue constructs with adipose tissue-derived microvascular fragments (ad-MVF) is an emerging pre-vascularisation strategy. Ad-MVF rapidly reassemble into new microvascular networks after in vivo implantation. Herein it was analysed whether this process was improved by erythropoietin (EPO). Ad-MVF were isolated from green fluorescent protein (GFP)+ as well as wild-type C57BL/6 mice and cultivated for 24 h in medium supplemented with EPO (20 IU/mL) or vehicle. Freshly isolated, non-cultivated ad-MVF served as controls. Protein expression, cell viability and proliferation of ad-MVF were assessed by proteome profiler array and fluorescence microscopy. GFP+ ad-MVF were seeded on collagen-glycosaminoglycan matrices, which were implanted into dorsal skinfold chambers of C57BL/6 mice, to analyse their vascularisation over 14 d by intravital fluorescence microscopy, histology and immunohistochemistry. Cultivation up-regulated the expression of pro- and anti-angiogenic factors within both vehicle- and EPO-treated ad-MVF when compared with non-cultivated controls. Moreover, EPO treatment suppressed cultivation-associated apoptosis and significantly increased the number of proliferating endothelial cells in ad-MVF when comp...Continue Reading

Citations

Nov 14, 2019·British Journal of Pharmacology·Maximilian M MengerEmmanuel Ampofo
Oct 22, 2019·Journal of Tissue Engineering·Matthias W LaschkeMichael D Menger
Aug 6, 2021·Aesthetic Plastic Surgery·F A Rodríguez-GarcíaQ L Torres-Salazar
Sep 19, 2021·Tissue Engineering and Regenerative Medicine·Thomas SpäterMatthias W Laschke
Nov 4, 2021·Tissue Engineering. Part B, Reviews·Matthias W Laschke, Michael D Menger

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