Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection

EMBO Reports
Diego BonettiMaria Pia Longhese


Homologous recombination requires nucleolytic degradation (resection) of DNA double-strand break (DSB) ends. In Saccharomyces cerevisiae, the MRX complex and Sae2 are involved in the onset of DSB resection, whereas extensive resection requires Exo1 and the concerted action of Dna2 and Sgs1. Here, we show that the checkpoint protein Rad9 limits the action of Sgs1/Dna2 in DSB resection by inhibiting Sgs1 binding/persistence at the DSB ends. When inhibition by Rad9 is abolished by the Sgs1-ss mutant variant or by deletion of RAD9, the requirement for Sae2 and functional MRX in DSB resection is reduced. These results provide new insights into how early and long-range resection is coordinated.


Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Keeney, N Kleckner
Jun 30, 2001·Molecular Cell·T UsuiJohn H J Petrini
Jun 1, 2007·Molecular Biology of the Cell·Valeria ViscardiMaria Pia Longhese
Aug 22, 2008·Nature·Pablo HuertasStephen P Jackson
Sep 23, 2008·Nature·Eleni P Mimitou, Lorraine S Symington
Oct 23, 2010·Molecular Cell·Alberto Ciccia, Stephen J Elledge
Aug 16, 2011·Nature Structural & Molecular Biology·Xuefeng ChenGrzegorz Ira
Aug 31, 2011·Molecular and Cellular Biology·Steven S FosterJohn H J Petrini
Sep 14, 2011·Annual Review of Genetics·Lorraine S Symington, Jean Gautier
Sep 11, 2012·Nature·Thomas CostelloeBertrand Llorente
Jan 29, 2013·Current Opinion in Genetics & Development·Andrew SeeberSusan M Gasser
Aug 15, 2014·Nucleic Acids Research·Greg H P NgoDavid Lydall


Oct 23, 2015·Nucleic Acids Research·Diego DibitettoAchille Pellicioli
Jan 19, 2016·Molecular Cell·Tatsuya KibeTitia de Lange
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Huan ChenLorraine S Symington
May 1, 2015·Nucleic Acids Research·Greg H P Ngo, David Lydall
Apr 22, 2016·Critical Reviews in Biochemistry and Molecular Biology·Lorraine S Symington
May 5, 2016·Cellular and Molecular Life Sciences : CMLS·Matteo VillaMaria Pia Longhese
Nov 20, 2015·PLoS Genetics·Elisa GobbiniMaria Pia Longhese
Oct 18, 2016·Molecular Cell·Julyun OhLorraine S Symington
Nov 24, 2018·Current Genetics·María Moriel-CarreteroBenjamin Pardo
Jun 22, 2018·The EMBO Journal·Elisa GobbiniMaria Pia Longhese
Dec 13, 2018·Genetics·Chiara Vittoria ColomboMaria Pia Longhese
Sep 24, 2019·Nucleic Acids Research·María Teresa VilloriaAndrés Clemente-Blanco
Feb 26, 2020·Genes·Julius Muellner, Kristina H Schmidt
Mar 22, 2018·Genetics·Marta Markiewicz-PotocznyDavid Lydall
Jun 25, 2019·Frontiers in Molecular Biosciences·Erika CasariMaria Pia Longhese
Sep 26, 2019·Nucleic Acids Research·Tai-Yuan YuLorraine S Symington
Apr 3, 2015·The Journal of Biological Chemistry·Xiaoqing ChenZhongsheng You
Apr 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ilaria CeppiPetr Cejka
Jun 25, 2020·Nature Communications·Matteo FerrariAchille Pellicioli
Dec 7, 2018·Genes·Julyun Oh, Lorraine S Symington
Sep 28, 2018·Frontiers in Genetics·Diego BonettiMaria Pia Longhese
Aug 6, 2019·Frontiers in Molecular Biosciences·Federica MariniAchille Pellicioli
Aug 12, 2018·Current Genetics·Hélène Bordelet, Karine Dubrana
Dec 5, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tai-Yuan YuLorraine S Symington
Mar 29, 2020·Biochemical Society Transactions·Chiara Vittoria ColomboMaria Pia Longhese

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