Escherichia coli aconitases and oxidative stress: post-transcriptional regulation of sodA expression

Microbiology
Yue TangJeffrey Green

Abstract

Escherichia coli possesses two aconitases, a stationary-phase enzyme (AcnA), which is induced by iron and oxidative stress, and a major but less stable enzyme (AcnB), synthesized during exponential growth. In addition to the catalytic activities of the holo-proteins, the apo-proteins function as post-transcriptional regulators by site-specific binding to acn mRNAs. Thus, it has been suggested that inactivation of the enzymes could mediate a rapidly reacting post-transcriptional component of the bacterial oxidative stress response. Here it is shown that E. coli acn mutants are hypersensitive to the redox-stress reagents H(2)O(2) and methyl viologen. Proteomic analyses further revealed that the level of superoxide dismutase (SodA) is enhanced in acnB and acnAB mutants, and by exposure to methyl viologen. The amounts of other proteins, including thioredoxin reductase, 2-oxoglutarate dehydrogenase, succinyl-CoA synthetase and chaperone proteins, were also affected in the acn mutants. The altered patterns of sodA expression were confirmed in studies with sodA-lacZ reporter strains. Quantitative Northern blotting indicated that AcnA enhances the stability of the sodA transcript, whereas AcnB lowers its stability. Direct evidence that...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J T GreenbergB Demple
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M W Hentze, L C Kühn
Feb 1, 1996·Microbiology·Alan J BradburyJohn R Guest
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·K Keyer, J A Imlay
Jan 1, 1997·Trends in Biochemical Sciences·M J GruerJ R Guest
Jun 20, 1997·The Journal of Biological Chemistry·G Richarme, T D Caldas
Jun 1, 1997·Microbiology·Megan J GruerJohn R Guest
Oct 6, 1997·The Journal of Biological Chemistry·P R GardnerA L Salzman
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·C Alén, A L Sonenshein
Nov 7, 1996·Chemical Reviews·Helmut BeinertC. David Stout

❮ Previous
Next ❯

Citations

Jan 7, 2016·Frontiers in Microbiology·Olga A PostnikovaLev G Nemchinov
Jul 22, 2010·Journal of Biotechnology·Meike Baumgart, Michael Bott
Mar 11, 2004·Molecular Microbiology·Yue TangJeffrey Green
Mar 25, 2011·Protein Science : a Publication of the Protein Society·Inoka C Perera, Anne Grove
Nov 3, 2012·Environmental Microbiology·Ewelina MichtaYvonne Mast
May 3, 2013·Microbiology·Othman A S BaothmanJeffrey Green
Feb 26, 2015·Plant Science : an International Journal of Experimental Plant Biology·Juan LiJian-Bo Yang
Feb 23, 2007·FEMS Microbiology Reviews·Stephen Spiro
Jul 29, 2006·Biochimica Et Biophysica Acta·Michelle L WallanderRichard S Eisenstein
Jul 25, 2012·Microbiological Research·Jeong Nam Kim, Young Min Kwon
Mar 1, 2012·FEMS Immunology and Medical Microbiology·Zhipeng ZhaiWei Zhang
Jul 9, 2010·FEMS Immunology and Medical Microbiology·Brian M PetersMark E Shirtliff
Aug 6, 2014·Nucleic Acids Research·Julie-Anna M Benjamin, Eric Massé
Nov 20, 2004·Nature Reviews. Microbiology·Jeffrey Green, Mark S Paget
Oct 3, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Dec 9, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Feb 2, 2018·Pathogens and Disease·Nicole GiordanoPaul E Carlson
Jan 14, 2012·Antioxidants & Redox Signaling·Jason C CrackNick E Le Brun
Sep 24, 2013·Journal of Bacteriology·Crystal M Austin, Robert J Maier
May 20, 2014·Journal of Bacteriology·James Patrick FolsomRoss P Carlson
Jul 15, 2015·Journal of Bacteriology·Crystal M AustinRobert J Maier
Apr 17, 2019·Life·Rafael Pernil, Enrico Schleiff
Apr 16, 2010·Canadian Journal of Physiology and Pharmacology·Julie-Anna M BenjaminEric Massé
Jun 5, 2003·The Journal of Biological Chemistry·Pedro EchaveJoaquim Ros
Dec 15, 2005·Journal of Bacteriology·Hyun-Jin KimAbraham L Sonenshein
Oct 13, 2009·Journal of Bacteriology·Uriel KoziolFrancisco Noya
Jan 15, 2008·Applied and Environmental Microbiology·Anne N ReidAlain Stintzi
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Mee-Jung Han, Sang Yup Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.