PMID: 108676Mar 1, 1979

Escherichia coli adenylate cyclase complex: regulation by the proton electrochemical gradient

Proceedings of the National Academy of Sciences of the United States of America
A Peterkofsky, C Gazdar

Abstract

Sugars such as glucose are transported into Escherichia coli by a coupled phosphorylation mechanism (the phosphoenolpyruvate:sugar phosphotransferase system, PTS). Transport of sugars through the PTS results in inhibition of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity by a mechanism involving a change in the state of phosphorylation of PTS proteins. Other sugars (e.g., lactose) are transported without modification by a mechanism involving proton cotransport, which requires a proton motive force across the cell membrane. We show here that uptake of sugars through the lactose transport system results in inhibition of adenylate cyclase activity if the proton symport mechanism is also active. The protonophore carbonyl cyanide m-chlorophenylhydrazone also inhibits adenylate cyclase activity. These data suggest that the steady-state electrochemical proton gradient regulates the activity of adenylate cyclase. We propose that sugar-dependent inhibition of adenylate cyclase activity may occur by either of two mechanisms. Sugars transported by the PTS inhibited adenylate cyclase activity by dephosphorylation of a regulatory protein, while sugars transported by the proton motive force system inhibit adenyl...Continue Reading

References

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·W J DobrogoszT Melton
Jan 1, 1981·Molecular & General Genetics : MGG·I H MajerfeldH V Rickenberg
Oct 18, 2006·Antonie van Leeuwenhoek·W N Konings
Jan 1, 1990·The International Journal of Biochemistry·S M StevensM I Chernin
Jul 18, 2002·Molecular Plant-microbe Interactions : MPMI·Margarita M Camacho CarvajalGuido V Bloemberg
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherP W Postma
Jan 14, 2012·BMC Microbiology·Raja VukantiLaura G Leff
May 27, 1980·Biochimica Et Biophysica Acta·I C West
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Oct 7, 2006·Prikladnaia biokhimiia i mikrobiologiia·S BaatoutM Mergeay
Jul 8, 2020·Antimicrobial Agents and Chemotherapy·M Ashley HudsonSteve W Lockless
Jun 1, 1987·Biotechnology and Bioengineering·N G RayK Venkatasubramanian

Related Concepts

Adenylate Cyclase
Lactogest
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Alkalescens-Dispar Group
Gluconeogenesis
Glucose, (beta-D)-Isomer
Lactates
Anhydrous lactose
Permease
Carbohydrate Metabolism

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