PMID: 1090755Apr 1, 1975

Escherichia coli capsule bacteriophages. IV. Free capsule depolymerase 29

Journal of Virology
W BesslerS Stirm

Abstract

The free host capsule depolymerase, induced by Escherichia coli capsule bacteriophage no. 29, and causing the formation of haloes around its plaques, has been purified to homogeneity. As judged from the following facts, this "enzyme" consists of free phage 29 spikes. (i) Detached phage organelles and depolymerase 29 particles exhibit the same molecular weight (about 245,000, as determined from the sedimentation equilibrium), contain polypeptide chains of the same two sizes (57,000 plus or minus 3,000 and 29,500 plus or minus 2,000, as determined by SDS-PAA gel electrophoresis), and have (within experimental error) the same sedimentation coefficient, isoelectric point, and amino acid composition. (ii) Isolated depolymerase and phage spikes in situ both catalyze the hydrolysis of glucosidic bonds in host capsular polysaccharide, leading ultimately to the formation of oligosaccharide fragments of one, two, and three hexasaccharide repeating units. (iii) Depolymerase 29 and phage 29 spikes have roughly the same electron optical dimensions. As tentatively estimated from the total and the virus-associated capsule depolymerase activity in the lysates, phage 29 infection seems to produce eight to seventeen times more free than incorpor...Continue Reading

Related Concepts

Alkalescens-Dispar Group
Microbial Anatomical Capsule Structure
SDS-PAGE
Capsular-polysaccharide galactohydrolase
Bacteriophages
Virus Replication
Bacteriophage Plaque Assay
Polysaccharides, Bacterial
Electron Microscopy
Senile Plaques

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