Escherichia coli CrfC Protein, a Nucleoid Partition Factor, Localizes to Nucleoid Poles via the Activities of Specific Nucleoid-Associated Proteins

Frontiers in Microbiology
Saki TaniguchiTsutomu Katayama

Abstract

The Escherichia coli CrfC protein is an important regulator of nucleoid positioning and equipartition. Previously we revealed that CrfC homo-oligomers bind the clamp, a DNA-binding subunit of the DNA polymerase III holoenzyme, promoting colocalization of the sister replication forks, which ensures the nucleoid equipartition. In addition, CrfC localizes at the cell pole-proximal loci via an unknown mechanism. Here, we demonstrate that CrfC localizes to the distinct subnucleoid structures termed nucleoid poles (the cell pole-proximal nucleoid-edges) even in elongated cells as well as in wild-type cells. Systematic analysis of the nucleoid-associated proteins (NAPs) and related proteins revealed that HU, the most abundant NAP, and SlmA, the nucleoid occlusion factor regulating the localization of cell division apparatus, promote the specific localization of CrfC foci. When the replication initiator DnaA was inactivated, SlmA and HU were required for formation of CrfC foci. In contrast, when the replication initiation was inhibited with a specific mutant of the helicase-loader DnaC, CrfC foci were sustained independently of SlmA and HU. H-NS, which forms clusters on AT-rich DNA regions, promotes formation of CrfC foci as well as tr...Continue Reading

References

Dec 1, 1996·Journal of Bacteriology·S G Addinall, J Lutkenhaus
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J PoglianoJ Beckwith
Apr 1, 1997·Molecular Microbiology·W Messer, C Weigel
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 29, 2000·The Journal of Biological Chemistry·S M ArfinG W Hatfield
Sep 6, 2000·Molecular Microbiology·U Von FreieslebenF G Hansen
Nov 28, 2000·Annual Review of Genetics·S Hiraga
Mar 30, 2001·Biochimie·M WeryJ Rouviere-Yaniv
Mar 12, 2002·Molecular Microbiology·Y SunakoS Hiraga
Apr 22, 2004·Nature Reviews. Microbiology·Charles J Dorman
Aug 28, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hidetaka OginoMotoyuki Sugai
Oct 8, 2004·The EMBO Journal·Michèle ValensFrédéric Boccard
Jan 20, 2006·The Journal of Biological Chemistry·Mike O'Donnell
Oct 19, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Taku OshimaNaotake Ogasawara
Jan 9, 2008·Molecular Microbiology·Sundari ChodavarapuJon M Kaguni
Feb 1, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Shun AdachiSota Hiraga
Feb 21, 2008·Molecular Microbiology·Sundari ChodavarapuJon M Kaguni
Mar 4, 2009·Plasmid·Torsten Waldminghaus, Kirsten Skarstad
Feb 9, 2010·Nature Reviews. Microbiology·Shane C Dillon, Charles J Dorman
Nov 26, 2010·Molecular Microbiology·Toshiyuki Hatano, Hironori Niki
Nov 30, 2010·The EMBO Journal·Nam Ky TonthatMaria A Schumacher
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hongbaek ChoThomas G Bernhardt
Mar 19, 2011·Molecular Microbiology·Oleksii SliusarenkoChristine Jacobs-Wagner
Aug 23, 2011·Current Opinion in Chemical Biology·Jon M Kaguni
Mar 1, 2012·Nucleic Acids Research·Torsten WaldminghausKirsten Skarstad

❮ Previous
Next ❯

Methods Mentioned

BETA
thermal
GTPase
flow cytometry

Software Mentioned

Microbe Tracker
MATLAB
ImageJ

Related Concepts