Escherichia coli cytochrome c nitrite reductase NrfA

Methods in Enzymology
T A ClarkeD J Richardson

Abstract

The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

References

Apr 19, 2002·The Journal of Biological Chemistry·Susannah R PoockDavid J Richardson
Sep 26, 2002·Journal of the American Chemical Society·Oliver EinsleFrank Neese
Apr 28, 2004·Bioelectrochemistry·James D GwyerJulea N Butt

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Citations

Aug 7, 2010·Bioinorganic Chemistry and Applications·Célia M SilveiraM Gabriela Almeida
Jan 30, 2016·BMC Microbiology·Tamara M KhlebodarovaVitaly A Likhoshvai
Nov 23, 2011·Biochemical Society Transactions·Colin W J LockwoodDavid J Richardson
Mar 15, 2019·The ISME Journal·Amy R SmithFrederick S Colwell
Jun 11, 2020·The Journal of Biological Chemistry·Julius CampeciñoEric L Hegg
Dec 5, 2013·The Journal of Biological Chemistry·Wouter J MaalckeBoran Kartal
Mar 3, 2021·The Journal of Biological Chemistry·Christina FerousiBoran Kartal
Feb 7, 2015·Journal of the American Chemical Society·Colin W J LockwoodJulea N Butt

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