Escherichia coli Dcu C4-dicarboxylate transporters dependent proton and potassium fluxes and FOF1-ATPase activity during glucose fermentation at pH 7.5.

Bioelectrochemistry
Lusine KarapetyanKaren Trchounian

Abstract

During fermentation in Escherichia coli succinate is transported via Dcu transporters, encoded dcuA, dcuB, dcuC and dcuD although the role of DcuD protein has not been elucidated yet. It has been shown contribution of Dcu transporters in the N,N'-dicyclohexylcarbodiimide (DCCD) sensitive proton and potassium transport through the cytoplasmic membrane and membrane-associated ATPase activity. Total H± efflux was decreased ~ 40% while K± uptake was absent in dcuD mutant. DCCD-sensitive H± flux was absent in dcuD nevertheless it was increased ~ 3 fold in dcuACB. K± uptake in dcuACB was stimulated ~ 30% compared to wild type but in DCCD assays K± ions were effluxed with the rate of 0.15 mmol/min per 109 cells/ml. In dcuACB mutant membrane potential (ΔΨ) was ~ 30 mV higher than in wild type. dcuD gene expression was increased in the dcuACB mutant respect to wild type at pH 7.5 (~120%), suggesting that an increment of DcuD activity compensates the lack of DcuA, DcuC and DcuB carriers. It can be concluded that active DcuD is important for H± efflux via the FOF1-ATPase and K± uptake at pH 7.5. In addition, DcuA, DcuB and DcuC transporters are crucial for regulating DCCD-sensitive K± transport and ΔΨ in E. coli.

References

Feb 1, 1977·Journal of Bacteriology·L W Adler, B P Rosen
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R OttoW N Konings
Jan 23, 2002·Biochimica Et Biophysica Acta·I G JanauschG Unden
May 11, 2005·Journal of Molecular Evolution·Pieter MonsieursKathleen Marchal
May 23, 2006·Biotechnology and Bioengineering·Yandi DharmadiRamon Gonzalez
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Sangjin Hong, Peter L Pedersen
Feb 16, 2010·Applied and Environmental Microbiology·Xueli ZhangLonnie O Ingram
Nov 28, 2012·Trends in Biotechnology·James M Clomburg, Ramon Gonzalez
Dec 29, 2012·Journal of Bioenergetics and Biomembranes·Karen TrchounianArmen Trchounian
Jan 3, 2013·Molecular Microbiology·Eleni KarinouArnaud Javelle
Dec 11, 2013·Applied Microbiology and Biotechnology·Jing ChenXueli Zhang
Jan 19, 2019·Trends in Biochemical Sciences·Armen Trchounian, Karen Trchounian

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