PMID: 7013806Mar 31, 1981Paper

Escherichia coli deoxyribonucleic acid dependent ribonucleic acid polymerase transcriptional pause sites on SV40 DNA F1

Biochemistry
R R Reisbig, J E Hearst

Abstract

We have studied elongation of SV40 DNA F1 by E. coli RNA polymerase looking specifically at the length of the transcript as a function of time. By running the transcription reactions at 18 degrees C with limited enzyme and adding heparin or rifampicin after elongation has started, we have achieved almost exclusive initiation from the SV40 DNA preferred promotor size [Zain, B. S., Weissmann, S. M., Lebowitz, P., & Lewis, A. M., Jr. (1973) J. Virol. 11, 682-693]. In the region within 1500 nucleotides of the initiation we observe nine prominent sites and a number of minor site where hesitation during elongation occurs. The positions of these hesitation points or pause sites are not effected by changes in the salt concentration, the simultaneous lowering of the concentrations of all the NTPs, or by increases in the RNA polymerase concentration, implying that the pause sites are a consequence of the RNA, DNA, and RNA polymerase ternary complex. The pause sites are not an artifact of the lowered temperature (18 degrees C) used in the experiments since they are also observed at 37 degrees C. The first four of these sites have been sequenced by using the 3'-O-methyl analogues of the ribonucleotide triphosphates. We have found no sequen...Continue Reading

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Citations

Apr 16, 1996·Biophysical Chemistry·P H von HippelK S Wilson
Jun 8, 2002·Journal of Molecular Biology·Ronald S Johnson, Rebecca E Chester
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maria L Kireeva, Mikhail Kashlev
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J GeiselmannP H von Hippel
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·J WuJ D Friesen
May 24, 1985·Nucleic Acids Research·W D MorganP H von Hippel
Dec 9, 1985·Nucleic Acids Research·S E LaFlammeD R Mills
Jan 1, 1997·Annual Review of Biochemistry·S M UptainM J Chamberlin
Jan 25, 1988·FEBS Letters·A SkorobogatyJ A Reiss
Oct 5, 1989·Journal of Molecular Biology·L S Folley, M Yarus
Apr 5, 1985·Journal of Molecular Biology·R F FisherC Yanofsky
Jul 17, 2019·Journal of Molecular Biology·Jin Young KangSeth A Darst

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