Escherichia coli diacylglycerol kinase: a case study in the application of solution NMR methods to an integral membrane protein

Biophysical Journal
O VinogradovaCharles R Sanders

Abstract

Diacylglycerol kinase (DAGK) is a 13-kDa integral membrane protein that spans the lipid bilayer three times and which is active in some micellar systems. In this work DAGK was purified using metal ion chelate chromatography, and its structural properties in micelles and organic solvent mixtures studies were examined, primarily to address the question of whether the structure of DAGK can be determined using solution NMR methods. Cross-linking studies established that DAGK is homotrimeric in decyl maltoside (DM) micelles and mixed micelles. The aggregate detergent-protein molecular mass of DAGK in both octyl glucoside and DM micelles was determined to be in the range of 100-110 kDa-much larger than the sum of the molecular weights of the DAGK trimers and the protein-free micelles. In acidic organic solvent mixtures, DAGK-DM complexes were highly soluble and yielded relatively well-resolved NMR spectra. NMR and circular dichroism studies indicated that in these mixtures the enzyme adopts a kinetically trapped monomeric structure in which it irreversibly binds several detergent molecules and is primarily alpha-helical, but in which its tertiary structure is largely disordered. Although these results provide new information regardin...Continue Reading

Citations

Apr 13, 1999·Bioorganic & Medicinal Chemistry·L P Liu, C M Deber
May 17, 2013·Nature·Dianfan LiMartin Caffrey
Aug 31, 2000·Analytical Biochemistry·L Czerski, C R Sanders
Jan 10, 2012·Annual Review of Biophysics·Wade D Van Horn, Charles R Sanders
Jan 1, 2013·Computational and Structural Biotechnology Journal·Amanda M Duran, Jens Meiler
Dec 29, 2009·Biochemical and Biophysical Research Communications·Young Pil KimYoung Ho Jeon
Nov 17, 2004·Journal of Molecular Biology·Mark Lorch, Paula J Booth
Oct 19, 2010·Biochimica Et Biophysica Acta·Satita TapaneeyakornAnthony Watts
Jan 21, 2014·Biochimica Et Biophysica Acta·Qingxin LiCongBao Kang
Oct 4, 2011·Biochemical and Biophysical Research Communications·Pan ShiChanglin Tian
Oct 28, 1998·Biophysical Journal·R S ProsserI V Shiyanovskaya
Jun 9, 2004·Chembiochem : a European Journal of Chemical Biology·Charles R SandersCharles D Ellis
Apr 3, 2015·Journal of Virology·Chia Min LeePei-Yong Shi
Jul 11, 2006·Magnetic Resonance in Chemistry : MRC·Charles R Sanders, Frank Sönnichsen
Apr 11, 2003·The Journal of Biological Chemistry·Anthony W PartridgeCharles M Deber
Dec 8, 2007·The Biochemical Journal·Isabel MéridaErnesto Merino
Mar 4, 2000·The Journal of Biological Chemistry·Y Zhou, J U Bowie
Oct 12, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·C M DeberC Wang
Sep 26, 2019·The Journal of Biological Chemistry·Robbins Puthenveetil, Olga Vinogradova
Mar 7, 2021·Communications Biology·Jianping LiJun Yang
Jul 25, 2014·Archives of Biochemistry and Biophysics·Jennifer NeumannDirk Schneider
Feb 21, 2002·Journal of the American Chemical Society·Paul A LuchetteCharles R Sanders
Sep 26, 2002·Journal of the American Chemical Society·Bonnie M GorzelleCharles R Sanders
Nov 25, 2000·Biochimica Et Biophysica Acta·C R Sanders, K Oxenoid
Apr 25, 2001·Biochemistry·K OxenoidC R Sanders
Jan 7, 2004·Biochemistry·Joanna K Nagy, Charles R Sanders
Oct 16, 2002·Biochemistry·Kirill OxenoidCharles R Sanders

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.