Escherichia coli DnaA interacts with HU in initiation at the E. coli replication origin

Molecular Microbiology
Sundari ChodavarapuJon M Kaguni

Abstract

Escherichia coli HU protein is a dimer encoded by two closely related genes whose expression is growth phase-dependent. As a major component of the bacterial nucleoid, HU binds to DNA non-specifically, but acts at the chromosomal origin (oriC) during initiation by stimulating strand opening in vitro. We show that the alpha dimer of HU is more active than other forms of HU in initiation of an oriC-containing plasmid because it more effectively promotes strand opening of oriC. Other results demonstrate that HU stabilizes the DnaA oligomer bound to oriC, and that the alpha subunit of HU interacts with the N-terminal region of DnaA. These observations support a model whereby DnaA interacts with the alpha dimer or the alphabeta heterodimer, depending on their cellular abundance, to recruit the respective form of HU to oriC. The greater activity of the alpha dimer of HU at oriC may stimulate initiation during early log phase compared with the lesser activity of the alphabeta heterodimer or the beta dimer.

References

Oct 15, 1979·FEBS Letters·J Rouvière-Yaniv, N O Kjeldgaard
Jul 1, 1989·Journal of Bacteriology·O HuismanJ Rouvière-Yaniv
Aug 30, 1996·The Journal of Biological Chemistry·K Tougu, K J Marians
Sep 12, 1997·The Journal of Biological Chemistry·M D Sutton, J M Kaguni
Mar 27, 2001·EMBO Reports·E BoyeK Skarstad
Sep 12, 2001·The Journal of Biological Chemistry·K M Carr, J M Kaguni
Jun 18, 2002·The Journal of Biological Chemistry·Mark Geanacopoulos, Sankar Adhya
Mar 14, 2003·Trends in Biochemical Sciences·Wlodzimierz Bujalowski
May 6, 2003·Journal of Bacteriology·Johanna Eltz CamaraElliott Crooke
Feb 6, 2004·Glycoconjugate Journal·Ronald J PattersonJohn L Wang
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kevin C McGarryAlan C Leonard
Sep 17, 2004·The Journal of Biological Chemistry·Magdalena M Felczak, Jon M Kaguni
Feb 3, 2005·Molecular Microbiology·Alan C Leonard, Julia E Grimwade
May 10, 2005·The Journal of Biological Chemistry·Magdalena M FelczakJon M Kaguni
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Cameron NeylonNicholas E Dixon
Jun 7, 2006·Annual Review of Microbiology·Jon M Kaguni

❮ Previous
Next ❯

Citations

Feb 4, 2011·Nucleic Acids Research·Sundari ChodavarapuJon M Kaguni
Nov 5, 2011·Nucleic Acids Research·Shogo Ozaki, Tsutomu Katayama
Aug 21, 2012·Nucleic Acids Research·Rafał DonczewAnna Zawilak-Pawlik
Aug 31, 2013·Nucleic Acids Research·Sundari ChodavarapuJon M Kaguni
May 29, 2010·Microbiology and Molecular Biology Reviews : MMBR·Andrew RobinsonNicholas E Dixon
Jun 7, 2011·Annual Review of Microbiology·Alan C Leonard, Julia E Grimwade
Jan 23, 2015·Frontiers in Microbiology·Marcin WolańskiJolanta Zakrzewska-Czerwińska
Aug 27, 2016·Frontiers in Molecular Biosciences·Katarzyna E WegrzynIgor Konieczny
Feb 5, 2010·Molecular Cell·Magdalena Makowska-Grzyska, Jon M Kaguni
Oct 18, 2016·Frontiers in Molecular Biosciences·Jyoti K JhaDhruba K Chattoraj
Jan 14, 2017·Genes·Matthew P MartinezDaniel L Kaplan
Sep 30, 2017·FEMS Microbiology Letters·Anteneh Hailu AbebeItzhak Fishov
Nov 9, 2017·MBio·Joanna HołówkaJolanta Zakrzewska-Czerwińska
Jan 10, 2018·Frontiers in Microbiology·Tsutomu KatayamaHironori Kawakami
Jan 28, 2009·Molecular Microbiology·Ingvild FlåttenKirsten Skarstad
Nov 19, 2011·FEMS Microbiology Reviews·Magdalena RajewskaIgor Konieczny
Jan 11, 2012·Bioscience, Biotechnology, and Biochemistry·Hideaki Nojiri
Dec 6, 2014·Acta Crystallographica. Section D, Biological Crystallography·Do Hee KimBong Jin Lee
Jul 4, 2019·The EMBO Journal·Tomas T RichardsonHeath Murray
Nov 13, 2019·Molecular Microbiology·Malgorzata Nowaczyk-CieszewskaAnna Magdalena Zawilak-Pawlik
Sep 18, 2007·Applied and Environmental Microbiology·Katarzyna Hupert-KocurekJon M Kaguni
Feb 18, 2011·Gut Microbes·Laurent Terradot, Anna Zawilak-Pawlik
Jan 6, 2016·The Journal of Biological Chemistry·Magdalena M FelczakJon M Kaguni
Mar 14, 2019·Frontiers in Microbiology·Gregory T MarczynskiPriya Patel
Feb 6, 2020·Journal of Basic Microbiology·Tingting HuangMorigen Moregen
Nov 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ganesh NatrajanLaurent Terradot
Apr 18, 2018·Frontiers in Microbiology·María-José FerrándizAdela G de la Campa
Mar 7, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yan Li, Hiroyuki Araki
Nov 3, 2020·Frontiers in Microbiology·Ana M Oliveira PaivaWiep Klaas Smits
Aug 1, 2009·EcoSal Plus·Akira Ishihama
Sep 1, 2010·EcoSal Plus·Alan C Leonard, Julia E Grimwade

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.