Escherichia coli engineered to produce eicosapentaenoic acid becomes resistant against oxidative damages

FEBS Letters
Takanori NishidaHidetoshi Okuyama

Abstract

The colony-forming ability of Escherichia coli genetically engineered to produce eicosapentaenoic acid (EPA) grown in 3mM hydrogen peroxide (H(2)O(2)) was similar to that of untreated cells. It was rapidly lost in the absence of EPA. H(2)O(2)-induced protein carbonylation was enhanced in cells lacking EPA. The fatty acid composition of the transformants was unaffected by H(2)O(2) treatment, but the amount of fatty acids decreased in cultures of cells lacking EPA and increased in cultures of cells producing EPA, suggesting that cellular EPA is stable in the presence of H(2)O(2) in vivo and may protect cells directly against oxidative damage. We discuss the possible role of EPA in partially blocking the penetration of H(2)O(2) into cells through membranes containing EPA.

References

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Feb 25, 2003·Bioscience, Biotechnology, and Biochemistry·Mina ArasekiKazuo Miyashita

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Citations

Aug 3, 2012·Applied Microbiology and Biotechnology·Swantje Behnken, Christian Hertweck
Sep 22, 2006·Biotechnology Letters·Yoshitake OrikasaHidetoshi Okuyama
Dec 11, 2007·Applied and Environmental Microbiology·Hidetoshi OkuyamaTakanori Nishida
Nov 26, 2010·Environmental Microbiology·Christine N Shulse, Eric E Allen
May 21, 2009·FEMS Microbiology Letters·Yoshitake OrikasaHidetoshi Okuyama

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