Escherichia coli Fiber Sensors Using Concentrated Dielectrophoretic Force with Optical Defocusing Method

ACS Sensors
Yi-Hsin TaiPei-Kuen Wei

Abstract

A sensitive tapered optical fiber tip combined with dielectrophoretic (DEP) trapping was used for rapid and label-free detection of bacteria in water. The angular spectrum of the optical field at the fiber tip was changed with the surrounding refractive index (RI). By measuring far-field intensity change at the defocus plane, the intensity sensitivity was up to 95 200%/RIU (RI unit), and the detection limit was 5.2 × 10-6 RIU at 0.5% intensity stability. By applying an AC voltage to a Ti/Al coated fiber tip and an indium-tin-oxide glass, the DEP force effectively trapped the Escherichia coli ( E. coli) near the fiber tip. Those bacteria can be directly measured from optical intensity change due to the increase of surrounding RI. By immobilizing the antibody on the Ti/Al fiber tip, the tests for specific K12 bacteria and nonspecific BL21 bacteria verified the specificity. The antibody-immobilized Ti/Al coated fiber tip with DEP trapping can detect bacteria at a concentration about 100 CFU/mL.

References

Sep 1, 1996·Analytical Chemistry·A P AbelH M Widmer
Jan 7, 2003·Biotechnology and Bioengineering·Tom T HuangMichael R Ladisch
May 26, 2006·Analytical and Bioanalytical Chemistry·Pavel TakmakovSergei Smirnov
Apr 7, 2010·Optics Letters·Yi-Hsin Tai, Pei-Kuen Wei
Nov 1, 2011·Analytical and Bioanalytical Chemistry·F BaldiniC Trono
May 15, 2012·Biosensors & Bioelectronics·Lin Lu, Soojin Jun
Sep 5, 2013·Biomicrofluidics·Dohyun Kim, Amy E Herr
Aug 5, 2014·Optics Express·Xuehao HuChristophe Caucheteur
Jan 27, 2015·Analytical and Bioanalytical Chemistry·Christophe CaucheteurJacques Albert
Jun 5, 2015·Biosensors & Bioelectronics·Daniel Quesada-González, Arben Merkoçi
Sep 1, 2015·Biosensors & Bioelectronics·Chen-Hsiang SangJeng-Tzong Sheu
Oct 31, 2015·The Analyst·Armando RicciardiAndrea Cusano

❮ Previous
Next ❯

Citations

Jan 1, 2021·The Review of Scientific Instruments·Mandeep Singh, Sanjeev Kumar Raghuwanshi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.