Abstract
In the current study, the virulence factors in Escherichia coli isolates from bovine mastitis were investigated, and the connection between these factors and infection was evaluated using phenotypic and genotypic analyses. Twenty-seven E. coli isolates were analyzed, and 2 were shown to produce verotoxin. All isolates had the ability to produce biofilms, although at different levels. One isolate was found to be sensitive to the bactericidal activity of bovine serum, 11 were intermediate, and 15 were resistant. Some isolates showed resistance to trimethoprim sulfa (9) and ampicillin (4), intermediate resistance to neomycin (1) and trimethoprim sulfa (5), and simultaneous resistance to ampicillin and trimethoprim sulfa (4). The fimH gene was found in all isolates and was associated with other virulence markers: pap (1), stb (8), cs31a (3), stb and vt2 (2), cs31a and stb (3), east1 and kps (1), stb and east1 (1), cs31a and east1 (1), and cs31a, stb, pap, and iucD (1). Serogroups were determined for 3 isolates: O93:H4, O83:H19, and O15:H11. Phylogenetic analysis showed that 23 isolates belonged to group A and 4 belonged to B1. The findings revealed that these E. coli isolates are opportunistic pathogens with different virulence fac...Continue Reading
References
Feb 1, 1992·Molecular Microbiology·J Yu, J B Kaper
Aug 30, 1991·Veterinary Microbiology·J NemethR Y Lo
Apr 11, 1989·Nucleic Acids Research·D A FidockP R Lehrbach
May 1, 1989·Veterinary Microbiology·P A Barrow, A W Hill
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M So, B J McCarthy
Oct 1, 1994·Journal of Clinical Microbiology·C SchultszJ Dankert
Apr 1, 1994·Canadian Journal of Microbiology·F DaigleP Lebel
Jun 1, 1994·Veterinary Microbiology·J NemethC L Gyles
Mar 1, 1994·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·B OjeniyiA Meyling
Feb 1, 1993·The Journal of Infectious Diseases·O SethabutrP Echeverria
Oct 1, 1995·FEMS Immunology and Medical Microbiology·S YamamotoO Yoshida
Apr 1, 1996·Infection and Immunity·T Yamamoto, P Echeverria
Jan 1, 1996·Journal of Dairy Science·W Fang, S Pyörälä
Nov 14, 1997·Journal of Clinical Microbiology·M BlancoJ Blanco
Feb 18, 1998·Journal of Clinical Microbiology·A W Paton, J C Paton
Mar 14, 1998·The Journal of Infectious Diseases·E BingenE Denamur
Jun 17, 1998·FEMS Microbiology Letters·Y BertinM Contrepois
Oct 10, 1998·Research in Microbiology·M BlancoJ Blanco
Dec 23, 1999·The Journal of Infectious Diseases·J R Johnson, A L Stell
Sep 30, 2000·Applied and Environmental Microbiology·O ClermontE Bingen
Apr 28, 2001·Journal of Clinical Microbiology·A J Bradley, M J Green
Jan 17, 2002·Veterinary Microbiology·T KaipainenS Pelkonen
Feb 2, 2002·Applied and Environmental Microbiology·Carl M SchroederJianghong Meng
May 31, 2002·Journal of Clinical Microbiology·Asis KhanG Balakrish Nair
Jun 29, 2002·Journal of Clinical Microbiology·Clifford G ClarkFrank G Rodgers
Oct 15, 2003·Veterinary Research·Henri SeegersFrançois Beaudeau
Oct 15, 2003·Veterinary Research·Christian BurvenichLuc Duchateau
Mar 12, 2004·Molecular Biology and Evolution·Patricia Escobar-PáramoErick Denamur
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
Apr 3, 2004·Letters in Applied Microbiology·S StepanovićM Svabić-Vlahović
Oct 2, 2004·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·A BeanR T Cursons
Jun 14, 2005·Research in Microbiology·Rob Van Houdt, Chris W Michiels
Jun 22, 2006·Veterinary Microbiology·Belgin DoganY H Schukken
Dec 12, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K KawanoY Goto
Feb 23, 2008·Current Opinion in Microbiology·Nahum Y ShpigelIlan Rosenshine
Jun 24, 2008·Veterinary Microbiology·S BlumG Leitner
Dec 7, 2010·Applied and Environmental Microbiology·Samantha E AllenClaire Jardine
Citations
Nov 10, 2013·Inflammation·Wen ZhangNaisheng Zhang
May 8, 2013·Canadian Journal of Microbiology·Vitor Oliveira SilvaMaria Aparecida Scatamburlo Moreira
Jul 6, 2014·Research in Veterinary Science·M A Ospina BarreroM A S Moreira
Jan 17, 2016·Pathogens and Disease·Fernanda GomesMariana Henriques
Jan 9, 2016·Journal of Wildlife Diseases·Sergio A ZanzaniMaria T Manfredi
Jan 30, 2015·Veterinary Microbiology·Julie-Hélène FairbrotherSerge Messier
Jun 26, 2012·Veterinary Microbiology·João Carlos Miguel CostaMaria Aparecida Scatamburlo Moreira
Apr 1, 2015·Jundishapur Journal of Microbiology·Zohreh HojatiAbolfazl Gholipour
Sep 4, 2015·PloS One·Shlomo E BlumGabriel Leitner
Jan 24, 2014·PloS One·Anja SipkaYnte Schukken
Jul 20, 2016·Mediators of Inflammation·Kai WangJian-Xin Liu
Jul 21, 2016·Scientific Reports·Robert J GoldstoneDavid G E Smith
Jan 25, 2018·Biomolecules·Steven G BieseckerÇağla Tükel
Oct 23, 2017·Journal of Dairy Science·Dexian ZhangMingchun Liu
Nov 5, 2017·Applied and Environmental Microbiology·Michael A OlsonDavid L Erickson
Apr 26, 2015·Applied and Environmental Microbiology·Elisabetta LambertiniAbani K Pradhan
Jul 27, 2014·Applied and Environmental Microbiology·Vitor O SilvaMaria Aparecida S Moreira
Jan 10, 2019·Foodborne Pathogens and Disease·Shelton E MurindaKayla Osmon
Oct 26, 2018·Tropical Animal Health and Production·Masoud MarashifardSeyed Sajjad Khoramrooz
Jan 11, 2019·Letters in Applied Microbiology·S T GuerraM G Ribeiro
Apr 1, 2020·Animals : an Open Access Journal From MDPI·Renata F RabelloAloysio M F Cerqueira
Jan 4, 2020·Veterinary Record Open·Magdalena Nüesch-InderbinenRoger Stephan
Jan 26, 2016·PloS One·Florent KempfPierre Germon
Feb 11, 2020·Drug Delivery·Samah Attia AlgharibShuyu Xie
May 10, 2017·BMC Genomics·Andreas LeimbachUlrich Dobrindt
Jun 20, 2017·PeerJ·Woranich HinthongNitaya Indrawattana
Feb 11, 2020·Journal of Dairy Science·Simony T GuerraMárcio G Ribeiro
Oct 17, 2020·Veterinary World·Zuhair Bani Ismail, Sameeh M Abutarbush
Aug 12, 2020·Asian-Australasian Journal of Animal Sciences·Wei Nee Cheng, Sung Gu Han
Dec 2, 2020·Animals : an Open Access Journal From MDPI·Maros CobirkaPetr Slama
Dec 17, 2020·Antibiotics·Priscila Luiza MelloMaria de Lourdes Ribeiro de Souza da Cunha
Mar 26, 2021·Frontiers in Microbiology·Yongqi MuWei Chen