Escherichia coli from clinical mastitis: serotypes and virulence factors

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
José Benedito C FernandesMaria Aparecida S Moreira

Abstract

In the current study, the virulence factors in Escherichia coli isolates from bovine mastitis were investigated, and the connection between these factors and infection was evaluated using phenotypic and genotypic analyses. Twenty-seven E. coli isolates were analyzed, and 2 were shown to produce verotoxin. All isolates had the ability to produce biofilms, although at different levels. One isolate was found to be sensitive to the bactericidal activity of bovine serum, 11 were intermediate, and 15 were resistant. Some isolates showed resistance to trimethoprim sulfa (9) and ampicillin (4), intermediate resistance to neomycin (1) and trimethoprim sulfa (5), and simultaneous resistance to ampicillin and trimethoprim sulfa (4). The fimH gene was found in all isolates and was associated with other virulence markers: pap (1), stb (8), cs31a (3), stb and vt2 (2), cs31a and stb (3), east1 and kps (1), stb and east1 (1), cs31a and east1 (1), and cs31a, stb, pap, and iucD (1). Serogroups were determined for 3 isolates: O93:H4, O83:H19, and O15:H11. Phylogenetic analysis showed that 23 isolates belonged to group A and 4 belonged to B1. The findings revealed that these E. coli isolates are opportunistic pathogens with different virulence fac...Continue Reading

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Citations

May 8, 2013·Canadian Journal of Microbiology·Vitor Oliveira SilvaMaria Aparecida Scatamburlo Moreira
Jan 17, 2016·Pathogens and Disease·Fernanda GomesMariana Henriques
Jan 9, 2016·Journal of Wildlife Diseases·Sergio A ZanzaniMaria T Manfredi
Jun 26, 2012·Veterinary Microbiology·João Carlos Miguel CostaMaria Aparecida Scatamburlo Moreira
Apr 1, 2015·Jundishapur Journal of Microbiology·Zohreh HojatiAbolfazl Gholipour
Sep 4, 2015·PloS One·Shlomo E BlumGabriel Leitner
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Jan 10, 2019·Foodborne Pathogens and Disease·Shelton E MurindaKayla Osmon
Apr 1, 2020·Animals : an Open Access Journal From MDPI·Renata F RabelloAloysio M F Cerqueira
Jan 4, 2020·Veterinary Record Open·Magdalena Nüesch-InderbinenRoger Stephan
Jan 26, 2016·PloS One·Florent KempfPierre Germon
Feb 11, 2020·Drug Delivery·Samah Attia AlgharibShuyu Xie
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Dec 2, 2020·Animals : an Open Access Journal From MDPI·Maros CobirkaPetr Slama

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Methods Mentioned

BETA
PCR

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