Escherichia coli heat shock protein DnaK: production and consequences in terms of monitoring cooking.

Applied and Environmental Microbiology
Karine SeyerLinda Saucier

Abstract

Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F(70)(10) of 1, 3, and 5 min), the intracellular concentration of DnaK in E. coli varied with the heating temperature (50 or 55 degrees C). In fact, the highest DnaK concentrations were found after treatments at the lower temperature (50 degrees C) applied for a longer time. Residual DnaK after heating was found to be necessary for cell recovery, and additional DnaK was produced during the recovery process. Overall, higher intracellular concentrations of DnaK tended to enhance cell resistance to a subsequent lethal stress. Indeed, E. coli cells that had undergone a sublethal heat shock (105 min at 55 degrees C, F(70)(10) = 3 min) accompanied by a 12-h recovery (containing 76,786 +/- 25,230 molecules/cell) resisted better than exponentially growing cells (38,500 +/- 6,056 molecules/cell) when later heated to 60 degrees C for 50 min (F(70)(10) = 5 min). Results reported here suggest that using stress protein to determine cell adaptation and surviv...Continue Reading

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Citations

May 16, 2007·Antimicrobial Agents and Chemotherapy·Maxim SuvorovShahriar Mobashery
Jun 28, 2011·Biochemical and Biophysical Research Communications·Qun Ma, Thomas K Wood
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ke ChenBernhard O Palsson

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