Escherichia coli K-12 Lacks a High-Affinity Assimilatory Cysteine Importer.

MBio
Yidan Zhou, James A Imlay

Abstract

The most direct route by which microbes might assimilate sulfur would be by importing cysteine. However, alone among the amino acids, cysteine does not have well-characterized importers. We determined that Escherichia coli can rapidly import cysteine, but in our experiments, it did so primarily through the LIV ATP-driven system that is dedicated to branched-chain amino acids. The affinity of this system for cysteine is far lower than for Leu, Ile, and Val, and so in their presence, cysteine is excluded. Thus, this transport is unlikely to be relevant in natural environments. Growth studies, transcriptomics, and transport assays failed to detect any high-affinity importer that is dedicated to cysteine assimilation. Enteric bacteria do not contain the putative cysteine importer that was identified in Campylobacter jejuni This situation is surprising, because E. coli deploys ion- and/or ATP-driven transporters that import cystine, the oxidized form of cysteine, with high affinity and specificity. We conjecture that in oxic environments, molecular oxygen oxidizes environmental cysteine to cystine, which E. coli imports. In anoxic environments where cysteine is stable, the cell chooses to assimilate hydrogen sulfide instead. Calcula...Continue Reading

References

Mar 1, 1979·Applied and Environmental Microbiology·J CarlssonM B Edlund
Jan 1, 1992·International Review of Cytology·S A Haney, D L Oxender
Jan 1, 1992·The Journal of Applied Bacteriology·G T MacfarlaneJ H Cummings
Oct 1, 1973·Journal of Bacteriology·J C Robbins, D L Oxender
Nov 1, 1968·Journal of Bacteriology·G F Ames, J R Roth
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Sep 19, 2000·Bioscience, Biotechnology, and Biochemistry·K MinoK Nakanishi
Jul 20, 2002·The Journal of Biological Chemistry·Ivan AhelDieter Söll
Apr 3, 2003·Experimental Biology and Medicine·Bart DeplanckeH Rex Gaskins
Jan 1, 1964·Archives of Biochemistry and Biophysics·G F AMES
Jan 2, 2004·Journal of Bacteriology·Takashi KoyanagiHidehiko Kumagai
Feb 20, 2004·Nature·Ertugrul M OzbudakAlexander Van Oudenaarden
Jul 8, 2005·Applied and Environmental Microbiology·Naoki AwanoHiroshi Takagi
Dec 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Patrick O'DonoghueZaida A Luthey-Schulten
Dec 6, 2008·Science·Ariel D Anbar
Apr 18, 2012·PloS One·Ernesto CuevasantaMatías N Möller
Sep 10, 2015·Journal of Bacteriology·Karin R Chonoles ImlayJames A Imlay
Nov 8, 2015·Nucleic Acids Research·Milton H SaierGabriel Moreno-Hagelsieb
Jan 5, 2016·PLoS Pathogens·James P R ConnollyAndrew J Roe
May 4, 2016·Journal of Bacteriology·Benjamin Julius Rauch, John J Perona
Jun 9, 2016·Nature Structural & Molecular Biology·Kaspar P Locher
May 11, 2017·MBio·Takahito MukaiDieter Söll

❮ Previous
Next ❯

Citations

Oct 19, 2021·Journal of Bacteriology·Achala Chittor, Karine A Gibbs

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
protein assay

Software Mentioned

BLAST

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.