Escherichia coli K1 invasion of human brain microvascular endothelial cells

Methods in Enzymology
Lip Nam Loh, Theresa H Ward

Abstract

The pathogenic Escherichia coli strain E. coli K1 is a primary causative agent of neonatal meningitis. Understanding how these bacteria cross the blood-brain barrier is vital to develop therapeutics. Here, we describe the use of live-cell imaging techniques to study E. coli K1 interactions with cellular markers following infection of human brain microvascular endothelial cells, a model system of the blood-brain barrier. We also discuss optimization of endothelial cell transfection conditions using nonviral transfection technique, bacterial labeling techniques, and in vitro assays to screen for fluorescent bacteria that retain their ability to invade host cells.

Citations

Nov 30, 2018·Journal of Cellular Biochemistry·Xuetao LuTianwen Gao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Related Papers

Cold Spring Harbor Protocols
Michael J RustXiaowei Zhuang
Cold Spring Harbor Protocols
Michael J RustXiaowei Zhuang
Annual Review of Physical Chemistry
Tie XiaXiaohong Fang
Essays in Biochemistry
Dhanya MullasseryMichael R H White
© 2022 Meta ULC. All rights reserved