Escherichia coli lipoyl synthase binds two distinct [4Fe-4S] clusters per polypeptide

Biochemistry
Robert M CicchilloSquire J Booker

Abstract

Lipoyl synthase (LS) is a member of a recently established class of metalloenzymes that use S-adenosyl-l-methionine (SAM) as the precursor to a high-energy 5'-deoxyadenosyl 5'-radical (5'-dA(*)). In the LS reaction, the 5'-dA(*) is hypothesized to abstract hydrogen atoms from C-6 and C-8 of protein-bound octanoic acid with subsequent sulfur insertion, generating the lipoyl cofactor. Consistent with this premise, 2 equiv of SAM is required to synthesize 1 equiv of the lipoyl cofactor, and deuterium transfer from octanoyl-d(15) H-protein of the glycine cleavage system-one of the substrates for LS-has been reported [Cicchillo, R. M., Iwig, D. F., Jones, A. D., Nesbitt, N. M., Baleanu-Gogonea, C., Souder, M. G., Tu, L., and Booker, S. J. (2004) Biochemistry 43, 6378-6386]. However, the exact identity of the sulfur donor remains unknown. We report herein that LS from Escherichia coli can accommodate two [4Fe-4S] clusters per polypeptide and that this form of the enzyme is relevant to turnover. One cluster is ligated by the cysteine amino acids in the C-X(3)-C-X(2)-C motif that is common to all radical SAM enzymes, while the other is ligated by the cysteine amino acids residing in a C-X(4)-C-X(5)-C motif, which is conserved only in l...Continue Reading

References

Aug 1, 1995·The Biochemical Journal·D E GreenJ R Guest
Feb 1, 1994·Biochemical Society Transactions·R DouceM Neuburger
Dec 23, 1998·FEBS Letters·B T BuiA Marquet
Jul 31, 1999·The Journal of Biological Chemistry·V RamamurthyE G Mueller
Apr 24, 2001·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·J Cheek, J B Broderick
Feb 28, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kirsty S HewitsonMarc Fontecave
Aug 28, 2002·Biochemical Society Transactions·J FrazzonD R Dean
Oct 10, 2002·FEBS Letters·Michele Mader CosperMichael K Johnson
Nov 21, 2002·Journal of the American Chemical Society·Michele Mader CosperMichael K Johnson
Dec 12, 2002·Biochemistry·Rüdiger BendaAlfred X Trautwein
Feb 20, 2003·Journal of Bacteriology·Sean W Jordan, John E Cronan
Jan 6, 2004·Science·Frederick BerkovitchCatherine L Drennan

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Citations

May 24, 2011·Applied Microbiology and Biotechnology·Ryota HideseNobuyoshi Esaki
Jun 2, 2006·Amino Acids·M A Grillo, S Colombatto
Apr 28, 2007·Photosynthesis Research·Fabrice RébeilléRoland Douce
Oct 24, 2012·Journal of the American Chemical Society·Kevin P McCuskerDanica Galonić Fujimori
Sep 3, 2013·Journal of the American Chemical Society·Bradley J LandgrafSquire J Booker
Feb 27, 2010·Journal of the American Chemical Society·Feng YanDanica Galonić Fujimori
Sep 28, 2007·Natural Product Reports·Peter H Buist
Sep 28, 2007·Natural Product Reports·Andrée MarquetMartin J Warren
Feb 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Brian P AntonRichard J Roberts
Sep 11, 2012·Journal of Bacteriology·Lauren D PalmerDiana M Downs
Mar 4, 2006·Journal of Bacteriology·Paul W KingMichael Seibert
Dec 19, 2007·Molecular and Cellular Biology·Cristy GellingUlrich Mühlenhoff
Dec 8, 2006·Annual Review of Plant Biology·Maria L GhirardiMichael Seibert
Feb 4, 2014·Archives of Biochemistry and Biophysics·Krista A Shisler, Joan B Broderick
Oct 28, 2008·Nature Chemical Biology·Abhishek ChatterjeeSteven E Ealick
Sep 21, 2012·Metallomics : Integrated Biometal Science·Rachel U Hutcheson, Joan B Broderick
May 2, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Marta K BruskaMarkus Reiher
Feb 13, 2016·Journal of the American Chemical Society·Bradley J Landgraf, Squire J Booker
Mar 1, 2008·Critical Reviews in Biochemistry and Molecular Biology·Perry A FreyFrank J Ruzicka
Jun 22, 2005·FEBS Letters·Sandrine Ollagnier-de ChoudensMarc Fontecave
Feb 4, 2016·Biochemistry·Nicholas D LanzSquire J Booker
Nov 21, 2015·Chemistry : a European Journal·Marta K BruskaMarkus Reiher
Nov 13, 2012·Current Opinion in Structural Biology·Krista A Shisler, Joan B Broderick
Aug 1, 2012·Biochimica Et Biophysica Acta·Nicholas D Lanz, Squire J Booker
Dec 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Aaron W FayYilin Hu
Jan 31, 2014·Chemical Reviews·Joan B BroderickEric M Shepard
Oct 16, 2007·Current Opinion in Chemical Biology·Squire J BookerTyler L Grove
Feb 13, 2007·Trends in Biochemical Sciences·Susan C Wang, Perry A Frey
Feb 6, 2007·Current Opinion in Chemical Biology·Michael R Leach, Deborah B Zamble
Dec 17, 2014·Biochimica Et Biophysica Acta·Maria-Eirini PandeliaCarsten Krebs
Jul 13, 2006·Angewandte Chemie·Paul DouglasPeter L Roach
Mar 17, 2010·Biochimica Et Biophysica Acta·J Kalervo HiltunenAlexander J Kastaniotis
Dec 29, 2013·Archives of Biochemistry and Biophysics·Kylie D Allen, Susan C Wang
May 6, 2016·Annual Review of Biochemistry·Bradley J LandgrafSquire J Booker
Oct 30, 2016·Metabolic Engineering·Gayle J BentleyFuzhong Zhang

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