PMID: 6988211Jan 1, 1980Paper

Escherichia coli murein transglycosylase. Purification by affinity chromatography and interaction with polynucleotides

European Journal of Biochemistry
W Kusser, U Schwarz

Abstract

Escherichia coli murein transglycosylase, a potential autolysin which splits the sugar chains of the murein sacculus, was rapidly purified from a crude cell extract by sequential chromatography on columns of blue Sepharose and poly(U)-Sepharose. In accordance with the binding to blue Sepharose and poly(U)-Sepharose, the transglycosylase is inhibited by Cibacron blue F3G-A, the affinity ligand of blue Sepharose, and also by polynucleotides, the latter, however, with varying efficiency. Among the polynucleotides tested, single-stranded DNA was found to be one of the most potent inhibitors. When bound to a blue Sepharose column, transglycosylase could be displaced from the column with single-stranded DNA. Taken together, these results point to a polynucleotide binding area on the transglycosylase molecule. Some aspects of the blue Sepharose affinity chromatography and the possible biological significance of the transglycosylase are discussed.

References

Dec 1, 1975·Journal of Bacteriology·J V HöltjeU Schwarz
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S T ThompsonE Stellwagen
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J De Maeyer-GuignardE De Maeyer
Sep 1, 1979·Journal of Bacteriology·W Keck, U Schwarz
Oct 15, 1975·European Journal of Biochemistry·J van HeijenoortY van Heijenoort
Feb 25, 1972·Nature·R HartmannU Schwarz
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·L M van Golde

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Citations

May 15, 1981·European Journal of Biochemistry·E H BeacheyU Schwarz
Oct 1, 1982·European Journal of Biochemistry·R Hakenbeck, M Kohiyama
May 2, 1985·European Journal of Biochemistry·W KeckU Schwarz
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Kelly C Rice, Kenneth W Bayles
Sep 6, 2005·Journal of Molecular Biology·Karin E van StraatenAndy-Mark W H Thunnissen
Apr 27, 2000·The Journal of Biological Chemistry·W Vollmer, A Tomasz
Sep 1, 1994·European Journal of Biochemistry·T Romeis, J V Höltje
Jan 1, 1982·Critical Reviews in Microbiology·B A Atkinson, L Amaral
Jun 24, 2017·Critical Reviews in Biochemistry and Molecular Biology·David A DikShahriar Mobashery
Jun 27, 1998·Journal of Bacteriology·A R KraftJ V Höltje
Aug 31, 2000·Analytical Biochemistry·N T Blackburn, A J Clarke
Mar 1, 1997·Journal of Bacteriology·W VollmerV Braun
Oct 1, 1981·Antimicrobial Agents and Chemotherapy·M GreensteinW M Maiese
Oct 1, 1980·Biochimica Et Biophysica Acta·K Bieńkowska-Szewczyk, A Taylor
Sep 1, 1991·Journal of Bacteriology·B Walderich, J V Höltje
Apr 20, 1990·Journal of Molecular Biology·H J RozeboomW Keck

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