Escherichia coli nitrate reductase subunit A: its role as the catalytic site and evidence for its modification.

Journal of Bacteriology
G R Chaudhry, C H MacGregor

Abstract

Subunits A and B were isolated from purified nitrate reductase by preparative electrophoresis in low levels of sodium dodecyl sulfate. Nonheme iron and low levels of molybdenum were associated with isolated subunit A but not with isolated subunit B. After dialysis against a source of molybdenum cofactor, subunit A regained tightly bound molybdenum and concomitantly regained enzyme activity and reactivity with anti-nitrate reductase antiserum. Subunit B neither bound cofactor nor regained activity or reactivity with antiserum. These data indicate that subunit A contains the active site of the enzyme. Subunit A was also found to be modified posttranslationally in a similar fashion as is subunit B. This was determined by comparison of partial proteolytic digests and amino acid analyses of A subunits from precursor and membrane-bound forms of nitrate reductase.

References

Mar 1, 1977·Bacteriological Reviews·B A Haddock, C W Jones
Nov 1, 1976·Journal of Bacteriology·R L Weiss
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R H Garrett, A Nason
May 16, 1972·Biochimica Et Biophysica Acta·R H Garrett
Mar 1, 1971·Journal of Bacteriology·R L Lester, J A DeMoss
Apr 1, 1981·Journal of Bacteriology·C S Hackett, C H MacGregor
Nov 1, 1981·Journal of Bacteriology·C H MacGregor, G E McElhaney
Jan 1, 1946·The Biochemical Journal·T W Goodwin, R A Morton

❮ Previous
Next ❯

Citations

Nov 1, 1986·Molecular & General Genetics : MGG·V BonnefoyM Chippaux
Jan 1, 1994·Antonie van Leeuwenhoek·V Bonnefoy, J A Demoss
Jul 1, 1986·Molecular & General Genetics : MGG·V BonnefoyM Chippaux
Apr 1, 1991·Journal of Bioenergetics and Biomembranes·A H Stouthamer
Oct 15, 1987·European Journal of Biochemistry·C IobbiG Giordano
Mar 30, 1990·European Journal of Biochemistry·C Iobbi-NivolG Giordano
Jul 9, 1999·Biochimica Et Biophysica Acta·L Philippot, O Højberg
Sep 1, 1984·Microbiological Reviews·W J Ingledew, R K Poole
Dec 1, 1990·Applied and Environmental Microbiology·L M WoodardP A Ketchum
Apr 1, 1991·Journal of Bacteriology·P A KetchumW J Payne
Jul 1, 1984·Journal of Bacteriology·S S RondeauJ A DeMoss

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.