Escherichia coli O antigen typing using DNA microarrays

Molecular and Cellular Probes
Yanhong Liu, Pina Fratamico

Abstract

DNA microarrays were developed for rapid identification of different serogroups of Escherichia coli in a single platform. Oligonucleotides, as well as PCR products from genes in the O antigen gene clusters of E. coli serogroups O7, O104, O111, and O157 were spotted onto glass slides. This was followed by hybridization with labeled long PCR products of the entire O antigen gene clusters of these serogroups. Results demonstrated that microarrays consisting of either oligonucleotides or PCR products generated specific signals for each serogroup. This is the first report describing the development of model DNA microarrays for determining the serogroup of E. coli strains.

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Citations

Apr 5, 2008·Analytical and Bioanalytical Chemistry·Andrew G GehringJeffrey D Brewster
Dec 11, 2008·Genes & Nutrition·Andrea Lauri, Paola O Mariani
Dec 14, 2011·Animal Health Research Reviews·Chitrita DebRoyPina M Fratamico
Jun 9, 2007·Journal of Microbiological Methods·Catherine YoshidaKris Rahn
May 21, 2009·FEMS Microbiology Reviews·Wenjun LiPierre-Edouard Fournier
May 21, 2016·Frontiers in Microbiology·Pina M FratamicoPeter Feng
Dec 23, 2015·Sensors·Andrew G GehringJoseph Uknalis
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Nov 30, 2019·FEMS Microbiology Reviews·Bin LiuGöran Widmalm
Feb 2, 2021·Frontiers in Microbiology·Jacob R ElderXianghe Yan

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