Escherichia coli O157:H7 biofilm formation on Romaine lettuce and spinach leaf surfaces reduces efficacy of irradiation and sodium hypochlorite washes

Journal of Food Science
Brendan A Niemira, Peter H Cooke

Abstract

Escherichia coli O157:H7 contamination of leafy green vegetables is an ongoing concern for consumers. Biofilm-associated pathogens are relatively resistant to chemical treatments, but little is known about their response to irradiation. Leaves of Romaine lettuce and baby spinach were dip inoculated with E. coli O157:H7 and stored at 4 degrees C for various times (0, 24, 48, 72 h) to allow biofilms to form. After each time, leaves were treated with either a 3-min wash with a sodium hypochlorite solution (0, 300, or 600 ppm) or increasing doses of irradiation (0, 0.25, 0.5, 0.75, or 1 kGy). Viable bacteria were recovered and enumerated. Chlorine washes were generally only moderately effective, and resulted in maximal reductions of 1.3 log CFU/g for baby spinach and 1.8 log CFU/g for Romaine. Increasing time in storage prior to chemical treatment had no effect on spinach, and had an inconsistent effect on 600 ppm applied to Romaine. Allowing time for formation of biofilm-like aggregations reduced the efficacy of irradiation. D(10) values (the dose required for a 1 log reduction) significantly increased with increasing storage time, up to 48 h postinoculation. From 0 h of storage, D(10) increased from 0.19 kGy to a maximum of 0.40 ...Continue Reading

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Citations

Jan 17, 2012·Applied and Environmental Microbiology·Ryan C FinkFrancisco Diez-Gonzalez
Aug 2, 2012·Food Microbiology·Amin N Olaimat, Richard A Holley
Jun 1, 2011·Journal of Food Science·Latha MurugesanAnuradha Prakash
Jul 15, 2009·Advances in Food and Nutrition Research·Keith WarrinerKari Dunfield
Feb 13, 2010·Current Opinion in Biotechnology·Faith J Critzer, Michael P Doyle
Aug 26, 2018·Journal of the Science of Food and Agriculture·Angela Laury-ShawMindy M Brashears
Aug 12, 2017·Critical Reviews in Food Science and Nutrition·Jae-Hyun Yoon, Sun-Young Lee
Jul 27, 2017·Current Microbiology·Sandra Folarin Oloketuyi, Fazlurrahman Khan
May 26, 2017·Journal of Food Science·Mohammad SadekuzzamanSang-Do Ha
May 7, 2019·Frontiers in Microbiology·Yulia KroupitskiShlomo Sela Saldinger
Dec 22, 2020·International Journal of Food Microbiology·Shiyun Yao, Haiqiang Chen
Mar 6, 2021·Critical Reviews in Food Science and Nutrition·Unji KimSe-Wook Oh

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