Escherichia coli O157:H7 survives within human macrophages: global gene expression profile and involvement of the Shiga toxins.

Infection and Immunity
Katherine PoirierFrance Daigle

Abstract

Escherichia coli O157:H7 is an important food-borne pathogen that specifically binds to the follicle-associated epithelium in the intestine, which rapidly brings this bacterial pathogen in contact with underlying human macrophages. Very little information is available about the interaction between E. coli O157:H7 and human macrophages. We evaluated the uptake and survival of strain EDL933 during infection of human macrophages. Surprisingly, EDL933 survived and multiplied in human macrophages at 24 h postinfection. The global gene expression profile of this pathogen during macrophage infection was determined. Inside human macrophages, upregulation of E. coli O157:H7 genes carried on O islands (such as pagC, the genes for both of the Shiga toxins, and the two iron transport system operons fit and chu) was observed. Genes involved in acid resistance and in the SOS response were upregulated. However, genes of the locus of enterocyte effacement or genes involved in peroxide resistance were not differentially expressed. Many genes with putative or unknown functions were upregulated inside human macrophages and may be newly discovered virulence factors. As the Shiga toxin genes were upregulated in macrophages, survival and cytotoxicit...Continue Reading

References

Jun 1, 1993·Journal of Bacteriology·K Z Abshire, F C Neidhardt
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·H OchmanE A Groisman
Jul 17, 1998·Clinical Microbiology Reviews·J C Paton, A W Paton
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
May 4, 1999·Infection and Immunity·C R Gentry-WeeksJ M Keith
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J E Graham, J E Clark-Curtiss
Apr 12, 2000·Molecular Microbiology·A R Fernández De HenestrosaR Woodgate
Feb 24, 2001·Nature·N T PernaF R Blattner
Oct 18, 2002·Nucleic Acids Research·Adel M TalaatStephen Albert Johnston
Oct 31, 2002·Gastroenterology·James E GrahamTimothy L Cover
Dec 12, 2002·Methods in Enzymology·France DaigleJosephine E Clark-Curtiss
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jovanka M VoyichFrank R DeLeo
Mar 26, 2003·Nucleic Acids Research·Steffen PorwollikMichael McClelland
Jan 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Partha MukhopadhyayGisela Storz
Jun 16, 2004·Current Opinion in Microbiology·Jay C D HintonMikael Rhen
Jun 26, 2004·Cell Structure and Function·O ShimadaS Atsumi
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jovanka M VoyichFrank R DeLeo
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Sylvia HeroldHerbert Schmidt
Mar 19, 2005·The Journal of Infectious Diseases·Lucilla BaldassarriGraziella Orefici
Mar 23, 2005·Lancet·Phillip I TarrWayne L Chandler
Jan 31, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sébastien P FaucherFrance Daigle

❮ Previous
Next ❯

Citations

Jan 11, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Alberto MedinaJosé A Orden
Mar 2, 2013·PloS One·Leticia V BentancorMarina S Palermo
May 28, 2010·Nihon saikingaku zasshi. Japanese journal of bacteriology·Takeshi Shimizu
Nov 19, 2011·Microbiology·Paul C M FoggHeather E Allison
Nov 21, 2012·FEMS Immunology and Medical Microbiology·Tarun BansalArul Jayaraman
Sep 12, 2013·Current Opinion in Microbiology·Disa L HammarlöfJay C D Hinton
Apr 16, 2013·FEMS Microbiology Letters·Samuel M ChekababJosée Harel
Jan 12, 2013·FEMS Microbiology Letters·Samuel Mohammed ChekababJosée Harel
May 4, 2010·Microbes and Infection·Birong LiSheryl S Justice
Jun 2, 2015·Nihon saikingaku zasshi. Japanese journal of bacteriology·Takeshi ShimizuMasanori Hatakeyama
Nov 10, 2011·Toxins·Stephanie Schüller
Dec 14, 2018·Frontiers in Microbiology·Ying HuaChengsong Wan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.