Escherichia coli outer membrane protease OmpT confers resistance to urinary cationic peptides

Microbiology and Immunology
Chang-Ye HuiSheng-He Huang

Abstract

Escherichia coli OmpT, located in the outer membrane, has been characterized as a plasminogen activator, with the ability to hydrolyze protamine and block its entry. In this investigation, a complex of low molecular weight cationic peptides purified from human urine by a combination of membrane ultrafiltration and weak cation exchange chromatography was characterized. The impact of OmpT on E. coli resistance to urinary cationic peptides was investigated by testing ompT knockout strains. The ompT mutants were more susceptible to urinary cationic peptides than ompT(+) strains, and this difference was abolished by complementation of the mutants with pUC19 carrying the ompT gene. The urinary protease inhibitor ulinastatin greatly decreased the resistance of the ompT(+) strains. Overall, the data indicate that OmpT may help E. coli persist longer in the urinary tract by enabling it to resist the antimicrobial activity of urinary cationic peptides.

References

Jun 2, 1995·The Journal of Biological Chemistry·C B WhiteP C Babbitt
May 16, 1998·The Journal of Clinical Investigation·E V ValoreT Ganz
Dec 13, 2000·The Journal of Biological Chemistry·C H ParkT Ganz
Jul 27, 2001·Biochemical and Biophysical Research Communications·J V VernekarV V Deshpande
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian C SchuttePaul B McCray
May 16, 2002·Infection and Immunity·Gillian MorrisonJulia Dorin
Aug 6, 2004·International Journal of Medical Microbiology : IJMM·Maini Kukkonen, Timo K Korhonen
Aug 20, 2004·Journal of Bacteriology·John D McCarterGeorge Georgiou
Feb 11, 2005·Postgraduate Medical Journal·R KucheriaN S Sheerin
Oct 19, 2007·Journal of the American Society of Nephrology : JASN·Michael Zasloff
May 12, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M-C WangJ-J Wu
Jul 8, 2009·International Journal of Molecular Sciences·Jin-Young KimYoonkyung Park

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Citations

Oct 12, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M C WangJ J Wu
May 12, 2011·Cellular and Molecular Life Sciences : CMLS·Tomaz Koprivnjak, Andreas Peschel
Nov 1, 2015·Journal of Medical Microbiology·Helen MiajlovicStephen Gj Smith
Jul 18, 2012·Critical Reviews in Microbiology·José Luis Anaya-LópezAlejandra Ochoa-Zarzosa
May 13, 2014·FEMS Microbiology Letters·Veena PremjaniJohn A Samis
Jul 28, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Xiaoxue Wang, Thomas K Wood
Nov 12, 2016·Applied Microbiology and Biotechnology·Nathaly Marín-MedinaChad Leidy
Apr 1, 2015·Infection and Immunity·John R BrannonHervé Le Moual
Nov 14, 2018·Infectious Disorders Drug Targets·Hassan ValadbeigiNourkhoda Sadeghifard
Oct 3, 2020·International Journal of Molecular Sciences·Damian NeubauerWojciech Kamysz
Mar 22, 2016·Microbiology Spectrum·Valerie P O'BrienScott J Hultgren

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